Simultaneous impedance spectroscopy and stimulation of human IPS-derived cardiac 3D spheroids in hanging-drop networks

Abstract

Here, we present electrical impedance spectroscopy (EIS) data of human iPS-derived cardiac 3D spheroids with electric stimulation integrated in a hanging drop network. Microscopy videos of the beating spheroids were correlated with synchronously obtained EIS recordings. For stimulation, the spheroid was exposed to a continuous sinusoidal electric field - in contrast to traditional pulse trains. This stimulating field was supplied via the same electrodes that were used for the EIS recordings. Our measurements revealed a beating frequency modulation upon tuning the stimulation signal amplitude.