Peracetic acid treatment generates potent inactivated oral vaccines from a broad range of culturable bacterial species
dc.contributor.author
Slack, Emma
dc.contributor.author
Moor, Kathrin
dc.contributor.author
Wotzka, Sandra Y.
dc.contributor.author
Toska, Albulena
dc.contributor.author
Diard, Médéric
dc.contributor.author
Hapfelmeier, Siegfried
dc.contributor.author
Slack, Emma
dc.date.accessioned
2019-07-17T06:39:36Z
dc.date.available
2017-06-12T01:20:49Z
dc.date.available
2019-07-17T06:39:36Z
dc.date.issued
2016-02-11
dc.identifier.issn
1664-3224
dc.identifier.other
10.3389/fimmu.2016.00034
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/113308
dc.identifier.doi
10.3929/ethz-b-000113308
dc.description.abstract
Our mucosal surfaces are the main sites of non-vector-borne pathogen entry, as well as the main interface with our commensal microbiota. We are still only beginning to understand how mucosal adaptive immunity interacts with commensal and pathogenic microbes to influence factors such as infectivity, phenotypic diversity, and within-host evolution. This is in part due to difficulties in generating specific mucosal adaptive immune responses without disrupting the mucosal microbial ecosystem itself. Here, we present a very simple tool to generate inactivated mucosal vaccines from a broad range of culturable bacteria. Oral gavage of 1010 peracetic acid-inactivated bacteria induces high-titer-specific intestinal IgA in the absence of any measurable inflammation or species invasion. As a proof of principle, we demonstrate that this technique is sufficient to provide fully protective immunity in the murine model of invasive non-typhoidal Salmonellosis, even in the face of severe innate immune deficiency.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers Media
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Oral vaccines
en_US
dc.subject
Inactivated vaccines
en_US
dc.subject
Salmonella typhimurium
en_US
dc.subject
Yersina enterocolytica
en_US
dc.subject
IgA
en_US
dc.title
Peracetic acid treatment generates potent inactivated oral vaccines from a broad range of culturable bacterial species
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Frontiers in Immunology
ethz.journal.volume
7
en_US
ethz.journal.abbreviated
Front Immunol
ethz.pages.start
34
en_US
ethz.size
14 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
010194167
ethz.publication.place
Lausanne
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::03589 - Hardt, Wolf-Dietrich / Hardt, Wolf-Dietrich
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::03589 - Hardt, Wolf-Dietrich / Hardt, Wolf-Dietrich
ethz.date.deposited
2017-06-12T01:23:11Z
ethz.source
ECIT
ethz.identifier.importid
imp593654251f19095306
ethz.ecitpid
pub:174995
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T12:53:30Z
ethz.rosetta.lastUpdated
2024-02-02T08:31:19Z
ethz.rosetta.versionExported
true
ethz.COinS
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