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dc.contributor.author
Bujak, Emil
dc.contributor.author
Ritz, Danilo
dc.contributor.author
Neri, Dario
dc.date.accessioned
2019-09-09T12:54:02Z
dc.date.available
2017-06-12T03:01:08Z
dc.date.available
2019-09-09T12:54:02Z
dc.date.issued
2015
dc.identifier.issn
2073-4468
dc.identifier.other
10.3390/antib4020071
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/114416
dc.identifier.doi
10.3929/ethz-b-000114416
dc.description.abstract
There is considerable interest in the characterization of novel tumor-associated antigens that lend themselves to antibody-mediated pharmacodelivery strategies. Delta-like 1 homolog protein (DLK1), which exists both as transmembrane protein and in soluble form, shows a restricted pattern of expression in healthy organs, while being overexpressed in some tumors. We have generated a human antibody specific to DLK1 using phage display technology. This reagent was used for a comprehensive characterization of DLK1 expression in freshly frozen sections of normal human adult tissues and of xenografted human tumors. DLK1 was virtually undetectable in most organs, except for placenta which was weakly positive. By contrast, DLK1 exhibited a moderate-to-strong expression in 8/9 tumor types tested. Our analysis shed light on previous conflicting reports on DLK1 expression in health and disease. The study suggests that DLK1 may be considered as a target for antibody-mediated pharmacodelivery strategies, in view of the protein’s limited expression in normal tissues and its abundance in the interstitium of neoplastic lesions
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
MDPI
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Pharmacodelivery
en_US
dc.subject
Immunostaining
en_US
dc.subject
DLK1
en_US
dc.subject
Phage display
en_US
dc.subject
Antibody
en_US
dc.subject
Tumor targeting
en_US
dc.subject
Small immune protein (SIP)
en_US
dc.title
A Monoclonal Antibody to Human DLK1 Reveals Differential Expression in Cancer and Absence in Healthy Tissues
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2015-04-16
ethz.journal.title
Antibodies
ethz.journal.volume
4
en_US
ethz.journal.issue
2
en_US
ethz.journal.abbreviated
Antibodies (Basel)
ethz.pages.start
71
en_US
ethz.pages.end
87
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
010151745
ethz.publication.place
Basel
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-12T03:02:24Z
ethz.source
ECIT
ethz.identifier.importid
imp5936543e274c184759
ethz.ecitpid
pub:176200
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-12T15:59:05Z
ethz.rosetta.lastUpdated
2019-09-09T12:54:15Z
ethz.rosetta.versionExported
true
ethz.COinS
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