In vivo visualization and quantification of collecting lymphatic vessel contractility using near-infrared imaging
Bachmann, Samia B.
Proulx, Steven T.
- Journal Article
Rights / licenseCreative Commons Attribution 4.0 International
Techniques to image lymphatic vessel function in either animal models or in the clinic are limited. In particular, imaging methods that can provide robust outcome measures for collecting lymphatic vessel function are sorely needed. In this study, we aimed to develop a method to visualize and quantify collecting lymphatic vessel function in mice, and to establish an in vivo system for evaluation of contractile agonists and antagonists using near-infrared fluorescence imaging. The flank collecting lymphatic vessel in mice was exposed using a surgical technique and a near-infrared tracer was infused into the inguinal lymph node. Collecting lymphatic vessel contractility and valve function could be easily visualized after the infusion. A diameter tracking method was established and the diameter of the vessel was found to closely correlate to near-infrared fluorescence signal. Phasic contractility measures of frequency and amplitude were established using an automated algorithm. The methods were validated by tracking the vessel response to topical application of a contractile agonist, prostaglandin F2α, and by demonstrating the potential of the technique for non-invasive evaluation of modifiers of lymphatic function. These new methods will enable high-resolution imaging and quantification of collecting lymphatic vessel function in animal models and may have future clinical applications. Show more
Journal / seriesScientific Reports
Pages / Article No.
PublisherNature Publishing Group
SubjectFluorescence imaging; Cardiovascular biology
Organisational unit03811 - Leroux, Jean-Christophe / Leroux, Jean-Christophe
03683 - Detmar, Michael / Detmar, Michael
130627 - Molecular mechanisms of angiogenesis and lymphangiogenesis in inflammation and cancer progression (SNF)
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