Show simple item record

dc.contributor.author
Chong, Chloe
dc.contributor.author
Scholkmann, Felix
dc.contributor.author
Bachmann, Samia B.
dc.contributor.author
Luciani, Paola
dc.contributor.author
Leroux, Jean-Christophe
dc.contributor.author
Detmar, Michael
dc.contributor.author
Proulx, Steven T.
dc.date.accessioned
2018-10-12T14:46:53Z
dc.date.available
2017-06-12T03:06:23Z
dc.date.available
2018-10-12T14:46:53Z
dc.date.issued
2016-03-10
dc.identifier.other
10.1038/srep22930
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/114447
dc.identifier.doi
10.3929/ethz-b-000114447
dc.description.abstract
Techniques to image lymphatic vessel function in either animal models or in the clinic are limited. In particular, imaging methods that can provide robust outcome measures for collecting lymphatic vessel function are sorely needed. In this study, we aimed to develop a method to visualize and quantify collecting lymphatic vessel function in mice, and to establish an in vivo system for evaluation of contractile agonists and antagonists using near-infrared fluorescence imaging. The flank collecting lymphatic vessel in mice was exposed using a surgical technique and a near-infrared tracer was infused into the inguinal lymph node. Collecting lymphatic vessel contractility and valve function could be easily visualized after the infusion. A diameter tracking method was established and the diameter of the vessel was found to closely correlate to near-infrared fluorescence signal. Phasic contractility measures of frequency and amplitude were established using an automated algorithm. The methods were validated by tracking the vessel response to topical application of a contractile agonist, prostaglandin F2α, and by demonstrating the potential of the technique for non-invasive evaluation of modifiers of lymphatic function. These new methods will enable high-resolution imaging and quantification of collecting lymphatic vessel function in animal models and may have future clinical applications.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Fluorescence imaging
en_US
dc.subject
Cardiovascular biology
en_US
dc.title
In vivo visualization and quantification of collecting lymphatic vessel contractility using near-infrared imaging
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Scientific Reports
ethz.journal.volume
6
en_US
ethz.pages.start
22930
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.notes
.
en_US
ethz.grant
Molecular mechanisms of angiogenesis and lymphangiogenesis in inflammation and cancer progression
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
006751867
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::03811 - Leroux, Jean-Christophe / Leroux, Jean-Christophe
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::03683 - Detmar, Michael / Detmar, Michael
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::03811 - Leroux, Jean-Christophe / Leroux, Jean-Christophe
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::03683 - Detmar, Michael / Detmar, Michael
ethz.grant.agreementno
130627
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projektförderung in Biologie und Medizin (Abteilung III)
ethz.date.deposited
2017-06-12T03:12:54Z
ethz.source
ECIT
ethz.identifier.importid
imp5936543ebc28486211
ethz.ecitpid
pub:176231
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-14T22:23:22Z
ethz.rosetta.lastUpdated
2019-01-02T14:30:58Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=In%20vivo%20visualization%20and%20quantification%20of%20collecting%20lymphatic%20vessel%20contractility%20using%20near-infrared%20imaging&rft.jtitle=Scientific%20Reports&rft.date=2016-03-10&rft.volume=6&rft.spage=22930&rft.au=Chong,%20Chloe&Scholkmann,%20Felix&Bachmann,%20Samia%20B.&Luciani,%20Paola&Leroux,%20Jean-Christophe&rft.genre=article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record