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dc.contributor.author
Reininger, Vanessa
dc.contributor.author
Schlegel, Markus
dc.date.accessioned
2018-10-04T13:03:20Z
dc.date.available
2017-06-12T03:20:04Z
dc.date.available
2018-10-04T13:03:20Z
dc.date.issued
2016-03-08
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0150591
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/114613
dc.identifier.doi
10.3929/ethz-b-000114613
dc.description.abstract
Background Phialocephala subalpina belongs to the Phialocephala fortinii s.l.–Acepphala applanata species complex (PAC) forming one of the major groups belonging to the dark septate endophytes (DSE). Depending on the strain, PAC was shown to form neutral to pathogenic associations with its host plant Picea abies. To understand PACs lifestyle we investigated the effect of presence/absence of Picea abies on the transcriptome of strain 6_70_1. Materials and Methods PAC strain 6_70_1 was grown in liquid Pachlewski media either induced by its host plant Picea abies or without host plant as a control. Mycelia were harvested in a time course (1, 2, 3, 4, 7, 11, 18 days) with and without induction by the host plant and the fungal transcriptome revealed by Illumina sequencing. Differential gene expression analysis over the time course comparing control and treatment at each time point using the ‘edgeR glm approach’ and a gene enrichment analysis using GO categories were performed. Results The three main functional groups within differentially expressed genes were ‘metabolism’, ‘transport’ and ‘cell rescue, defense and virulence’. Additionally, genes especially involved in iron metabolism could be detected by gene set enrichment analysis. Conclusion In conclusion, we found PAC strain 6_70_1 to be metabolically very active during colonization of its host plant Picea abies. A major shift in functional groups over the time course of this experiment could not be observed but GO categories which were found to be enriched showed different emphasis depending in the day post induction.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Analysis of the Phialocephala subalpina Transcriptome during Colonization of Its Host Plant Picea abies
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
PLoS ONE
ethz.journal.volume
11
en_US
ethz.journal.issue
3
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e0150591
en_US
ethz.size
16 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
006206116
ethz.publication.place
San Francisco, CA, USA
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-12T03:21:20Z
ethz.source
ECIT
ethz.identifier.importid
imp593654424cc1536493
ethz.ecitpid
pub:176411
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T18:40:38Z
ethz.rosetta.lastUpdated
2018-10-04T13:03:32Z
ethz.rosetta.versionExported
true
ethz.COinS
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