Zur Kurzanzeige

dc.contributor.author
Prats Mateu, Batirtze
dc.contributor.author
Hauser, Marie Theres
dc.contributor.author
Heredia, Antonio
dc.contributor.author
Gierlinger, Notburga
dc.date.accessioned
2019-09-12T12:20:29Z
dc.date.available
2017-06-12T03:55:25Z
dc.date.available
2019-09-12T11:12:04Z
dc.date.available
2019-09-12T12:20:29Z
dc.date.issued
2016
dc.identifier.other
10.3389/fchem.2016.00010
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/114978
dc.identifier.doi
10.3929/ethz-b-000114978
dc.description.abstract
Waterproofing of the aerial organs of plants imposed a big evolutionary step during the colonization of the terrestrial environment. The main plant polymers responsible of water repelling are lipids and lignin, which play also important roles in the protection against biotic/abiotic stresses, regulation of flux of gases and solutes, and mechanical stability against negative pressure, among others. While the lipids, non-polymerized cuticular waxes together with the polymerized cutin, protect the outer surface, lignin is confined to the secondary cell wall within mechanical important tissues. In the present work a micro cross-section of the stem of Arabidopsis thaliana was used to track in situ the distribution of these non-carbohydrate polymers by Confocal Raman Microscopy. Raman hyperspectral imaging gives a molecular fingerprint of the native waterproofing tissues and cells with diffraction limited spatial resolution (~300 nm) at relatively high speed and without any tedious sample preparation. Lipids and lignified tissues as well as their effect on water content was directly visualized by integrating the 1299, 1600, and 3400 cm−1 band, respectively. For detailed insights into compositional changes of these polymers vertex component analysis was performed on selected sample positions. Changes have been elucidated in the composition of lignin within the lignified tissues and between interfascicular fibers and xylem vessels. Hydrophobizing changes were revealed from the epidermal layer to the cuticle as well as a change in the aromatic composition within the cuticle of trichomes. To verify Raman signatures of different waterproofing polymers additionally Raman spectra of the cuticle and cutin monomer from tomato (Solanum lycopersicum) as well as aromatic model polymers (milled wood lignin and dehydrogenation polymer of coniferyl alcohol) and phenolic acids were acquired.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers Media
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Lignin
en_US
dc.subject
Cutin
en_US
dc.subject
Wax
en_US
dc.subject
Raman
en_US
dc.subject
Cuticle
en_US
dc.subject
Secondary cell wall
en_US
dc.subject
Trichomes
en_US
dc.title
Waterproofing in Arabidopsis: Following Phenolics and Lipids In situ by Confocal Raman Microscopy
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2016-02-29
ethz.journal.title
Frontiers in Chemistry
ethz.journal.volume
4
en_US
ethz.pages.start
10
en_US
ethz.size
13 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
010260777
ethz.publication.place
Lausanne
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-12T03:59:11Z
ethz.source
ECIT
ethz.identifier.importid
imp5936544a2536764296
ethz.ecitpid
pub:176788
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-15T04:13:46Z
ethz.rosetta.lastUpdated
2019-09-12T12:20:44Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Waterproofing%20in%20Arabidopsis:%20Following%20Phenolics%20and%20Lipids%20In%20situ%20by%20Confocal%20Raman%20Microscopy&rft.jtitle=Frontiers%20in%20Chemistry&rft.date=2016&rft.volume=4&rft.spage=10&rft.au=Prats%20Mateu,%20Batirtze&Hauser,%20Marie%20Theres&Heredia,%20Antonio&Gierlinger,%20Notburga&rft.genre=article&
 Suchen via SFX

Dateien zu diesem Eintrag

Thumbnail

Publikationstyp

Zur Kurzanzeige