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dc.contributor.author
Turnbull, Lynne
dc.contributor.author
Toyofuku, Masanori
dc.contributor.author
Hynen, Amelia L.
dc.contributor.author
Kurosawa, Masaharu
dc.contributor.author
Pessi, Gabriella
dc.contributor.author
Osvath, Sarah R.
dc.contributor.author
Cárcamo-Oyarce, Gerardo
dc.contributor.author
Gloag, Erin S.
dc.contributor.author
Shimoni, Raz
dc.contributor.author
Omasits, Ulrich
dc.contributor.author
Ito, Satoshi
dc.contributor.author
Yap, Xinhui
dc.contributor.author
Monahan, Leigh G.
dc.contributor.author
Cavaliere, Rosalia
dc.contributor.author
Ahrens, Christian H.
dc.contributor.author
Charles, Ian G.
dc.contributor.author
Eberl, Leo
dc.contributor.author
Whitchurch, Cynthia B.
dc.date.accessioned
2018-09-07T10:38:57Z
dc.date.available
2017-06-12T05:08:32Z
dc.date.available
2018-09-07T10:38:57Z
dc.date.issued
2016
dc.identifier.other
10.1038/ncomms11220
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/115837
dc.identifier.doi
10.3929/ethz-b-000115837
dc.description.abstract
Many bacteria produce extracellular and surface-associated components such as membrane vesicles (MVs), extracellular DNA and moonlighting cytosolic proteins for which the biogenesis and export pathways are not fully understood. Here we show that the explosive cell lysis of a sub-population of cells accounts for the liberation of cytosolic content in Pseudomonas aeruginosa biofilms. Super-resolution microscopy reveals that explosive cell lysis also produces shattered membrane fragments that rapidly form MVs. A prophage endolysin encoded within the R- and F-pyocin gene cluster is essential for explosive cell lysis. Endolysin-deficient mutants are defective in MV production and biofilm development, consistent with a crucial role in the biogenesis of MVs and liberation of extracellular DNA and other biofilm matrix components. Our findings reveal that explosive cell lysis, mediated through the activity of a cryptic prophage endolysin, acts as a mechanism for the production of bacterial MVs.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Explosive cell lysis as a mechanism for the biogenesis of bacterial membrane vesicles and biofilms
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2016-04-14
ethz.journal.title
Nature Communications
ethz.journal.volume
7
en_US
ethz.pages.start
11220
en_US
ethz.size
13 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
007044158
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::03853 - Sturla, Shana / Sturla, Shana
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::03853 - Sturla, Shana / Sturla, Shana
ethz.date.deposited
2017-06-12T05:15:17Z
ethz.source
ECIT
ethz.identifier.importid
imp5936545ad5fc824325
ethz.ecitpid
pub:177674
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-26T04:07:47Z
ethz.rosetta.lastUpdated
2019-02-03T05:27:05Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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