Show simple item record

dc.contributor.author
Böhm, Alex
dc.contributor.author
Arnoldini, Markus
dc.contributor.author
Bergmiller, Tobias
dc.contributor.author
Röösli, Thomas
dc.contributor.author
Bigosch, Colette
dc.contributor.author
Ackermann, Martin
dc.date.accessioned
2018-10-31T16:50:15Z
dc.date.available
2017-06-12T05:37:31Z
dc.date.available
2018-10-31T16:50:15Z
dc.date.issued
2016-04-19
dc.identifier.issn
1553-7390
dc.identifier.issn
1553-7404
dc.identifier.other
10.1371/journal.pgen.1005974
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/116133
dc.identifier.doi
10.3929/ethz-b-000116133
dc.description.abstract
In bacteria, replicative aging manifests as a difference in growth or survival between the two cells emerging from division. One cell can be regarded as an aging mother with a decreased potential for future survival and division, the other as a rejuvenated daughter. Here, we aimed at investigating some of the processes involved in aging in the bacterium Escherichia coli, where the two types of cells can be distinguished by the age of their cell poles. We found that certain changes in the regulation of the carbohydrate metabolism can affect aging. A mutation in the carbon storage regulator gene, csrA, leads to a dramatically shorter replicative lifespan; csrA mutants stop dividing once their pole exceeds an age of about five divisions. These old-pole cells accumulate glycogen at their old cell poles; after their last division, they do not contain a chromosome, presumably because of spatial exclusion by the glycogen aggregates. The new-pole daughters produced by these aging mothers are born young; they only express the deleterious phenotype once their pole is old. These results demonstrate how manipulations of nutrient allocation can lead to the exclusion of the chromosome and limit replicative lifespan in E. coli, and illustrate how mutations can have phenotypic effects that are specific for cells with old poles. This raises the question how bacteria can avoid the accumulation of such mutations in their genomes over evolutionary times, and how they can achieve the long replicative lifespans that have recently been reported.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science (PLoS)
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Genetic Manipulation of Glycogen Allocation Affects Replicative Lifespan in E. coli
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
PLoS Genetics
ethz.journal.volume
12
en_US
ethz.journal.issue
4
en_US
ethz.journal.abbreviated
PLoS Genet
ethz.pages.start
e1005974
en_US
ethz.size
17 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
005410305
ethz.publication.place
San Francisco, CA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02721 - Inst. f. Biogeochemie u. Schadstoffdyn. / Inst. Biogeochem. and Pollutant Dynamics::03743 - Ackermann, Martin / Ackermann, Martin
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02721 - Inst. f. Biogeochemie u. Schadstoffdyn. / Inst. Biogeochem. and Pollutant Dynamics::03743 - Ackermann, Martin / Ackermann, Martin
ethz.date.deposited
2017-06-12T05:44:40Z
ethz.source
ECIT
ethz.identifier.importid
imp59365460c0d2d32709
ethz.ecitpid
pub:177989
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-26T20:06:48Z
ethz.rosetta.lastUpdated
2021-02-15T02:21:31Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Genetic%20Manipulation%20of%20Glycogen%20Allocation%20Affects%20Replicative%20Lifespan%20in%20E.%20coli&rft.jtitle=PLoS%20Genetics&rft.date=2016-04-19&rft.volume=12&rft.issue=4&rft.spage=e1005974&rft.issn=1553-7390&1553-7404&rft.au=B%C3%B6hm,%20Alex&Arnoldini,%20Markus&Bergmiller,%20Tobias&R%C3%B6%C3%B6sli,%20Thomas&Bigosch,%20Colette&rft.genre=article&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record