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dc.contributor.author
Sgier, Linn
dc.contributor.author
Freimann, Remo
dc.contributor.author
Zupanic, Anze
dc.contributor.author
Kroll, Alexandra
dc.date.accessioned
2018-09-13T14:20:56Z
dc.date.available
2017-06-12T05:51:44Z
dc.date.available
2018-09-13T14:20:56Z
dc.date.issued
2016
dc.identifier.issn
2041-1723
dc.identifier.other
10.1038/ncomms11587
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/116326
dc.identifier.doi
10.3929/ethz-b-000116326
dc.description.abstract
Biofilms serve essential ecosystem functions and are used in different technical applications. Studies from stream ecology and waste-water treatment have shown that biofilm functionality depends to a great extent on community structure. Here we present a fast and easy-to-use method for individual cell-based analysis of stream biofilms, based on stain-free flow cytometry and visualization of the high-dimensional data by viSNE. The method allows the combined assessment of community structure, decay of phototrophic organisms and presence of abiotic particles. In laboratory experiments, it allows quantification of cellular decay and detection of survival of larger cells after temperature stress, while in the field it enables detection of community structure changes that correlate with known environmental drivers (flow conditions, dissolved organic carbon, calcium) and detection of microplastic contamination. The method can potentially be applied to other biofilm types, for example, for inferring community structure for environmental and industrial research and monitoring.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Flow cytometry combined with viSNE for the analysis of microbial biofilms and detection of microplastics
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2016-05-18
ethz.journal.title
Nature Communications
ethz.journal.volume
7
en_US
ethz.journal.abbreviated
Nat Commun
ethz.pages.start
11587
en_US
ethz.size
10 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
007044158
ethz.publication.place
London
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::03978 - Wutz, Anton / Wutz, Anton
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::03978 - Wutz, Anton / Wutz, Anton
ethz.date.deposited
2017-06-12T05:56:24Z
ethz.source
ECIT
ethz.identifier.importid
imp59365463d076121555
ethz.ecitpid
pub:178185
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-18T12:32:44Z
ethz.rosetta.lastUpdated
2024-02-02T06:06:34Z
ethz.rosetta.versionExported
true
ethz.COinS
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