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dc.contributor.author
Degrauwe, Nils
dc.contributor.author
Schlumpf, Tommy B.
dc.contributor.author
Janiszewska, Michalina
dc.contributor.author
Martin, Patricia
dc.contributor.author
Cauderay, Alexandra
dc.contributor.author
Provero, Paolo
dc.contributor.author
Riggi, Nicolo
dc.contributor.author
Suvà, Maria-L.
dc.contributor.author
Paro, Renato
dc.contributor.author
Stamenkovic, Ivan
dc.date.accessioned
2018-09-17T16:10:45Z
dc.date.available
2017-06-12T06:08:14Z
dc.date.available
2018-09-17T16:10:45Z
dc.date.issued
2016-05
dc.identifier.other
10.1016/j.celrep.2016.04.086
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/116536
dc.identifier.doi
10.3929/ethz-b-000116536
dc.description.abstract
Cancer stem cells (CSCs) can drive tumor growth, and their maintenance may rely on post-transcriptional regulation of gene expression, including that mediated by microRNAs (miRNAs). The let-7 miRNA family has been shown to induce differentiation by silencing stem cell programs. Let-7-mediated target gene suppression is prevented by LIN28A/B, which reduce let-7 biogenesis in normal embryonic and some cancer stem cells and ensure maintenance of stemness. Here, we find that glioblastoma stem cells (GSCs) lack LIN28 and express both let-7 and their target genes, suggesting LIN28-independent protection from let-7 silencing. Using photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP), we show that insulin-like growth factor 2 mRNA-binding protein 2 (IMP2) binds to let-7 miRNA recognition elements (MREs) and prevents let-7 target gene silencing. Our observations define the RNA-binding repertoire of IMP2 and identify a mechanism whereby it supports GSC and neural stem cell specification.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Elsevier
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
The RNA Binding Protein IMP2 Preserves Glioblastoma Stem Cells by Preventing let-7 Target Gene Silencing
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2016-05-12
ethz.journal.title
Cell Reports
ethz.journal.volume
15
en_US
ethz.journal.issue
8
en_US
ethz.pages.start
1634
en_US
ethz.pages.end
1647
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
007612702
ethz.publication.place
New York, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03748 - Paro, Renato (emeritus) / Paro, Renato (emeritus)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03748 - Paro, Renato (emeritus) / Paro, Renato (emeritus)
ethz.date.deposited
2017-06-12T06:14:25Z
ethz.source
ECIT
ethz.identifier.importid
imp593654673eb8f56186
ethz.ecitpid
pub:178409
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-18T09:53:44Z
ethz.rosetta.lastUpdated
2018-11-08T02:28:12Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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