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dc.contributor.author
Fussenegger, Martin
dc.contributor.author
Saxena, Pratik
dc.contributor.author
Dobrin, Anton
dc.date.accessioned
2021-10-15T07:41:30Z
dc.date.available
2017-06-12T06:40:59Z
dc.date.available
2021-10-15T07:41:30Z
dc.date.issued
2016
dc.identifier.issn
1757-9694
dc.identifier.issn
1757-9708
dc.identifier.other
10.1039/c5ib00263j
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/116883
dc.identifier.doi
10.3929/ethz-b-000116883
dc.description.abstract
Synthetic biology, an engineering, circuit-driven approach to biology, has developed whole new classes of therapeutics. Unfortunately, these advances have thus far been undercapitalized upon by basic researchers. As discussed herein, using synthetic circuits, one can undertake exhaustive investigations of the endogenous circuitry found in nature, develop novel detectors and better temporally and spatially controlled inducers. One could detect changes in DNA, RNA, protein or even transient signaling events, in cell-based systems, in live mice, and in humans. Synthetic biology has also developed inducible systems that can be induced chemically, optically or using radio waves. This induction has been re-wired to lead to changes in gene expression, RNA stability and splicing, protein stability and splicing, and signaling via endogenous pathways. Beyond simple detectors and inducible systems, one can combine these modalities and develop novel signal integration circuits that can react to a very precise pre-programmed set of conditions or even to multiple sets of precise conditions. In this review, we highlight some tools that were developed in which these circuits were combined such that the detection of a particular event automatically triggered a specific output. Furthermore, using novel circuit-design strategies, circuits have been developed that can integrate multiple inputs together in Boolean logic gates composed of up to 6 inputs. We highlight the tools available and what has been developed thus far, and highlight how some clinical tools can be very useful in basic science. Most of the systems that are presented can be integrated together; and the possibilities far exceed the number of currently developed strategies.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Royal Society of Chemistry
en_US
dc.rights.uri
http://rightsstatements.org/page/InC-NC/1.0/
dc.title
Synthetic biology: Applying biological circuits beyond novel therapies
en_US
dc.type
Review Article
dc.rights.license
In Copyright - Non-Commercial Use Permitted
dc.date.published
2015-12-24
ethz.journal.title
Integrative Biology
ethz.journal.volume
8
en_US
ethz.journal.issue
4
en_US
ethz.journal.abbreviated
Integr. biol. (Print)
ethz.pages.start
409
en_US
ethz.pages.end
430
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.notes
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.scopus
ethz.identifier.nebis
005750211
ethz.publication.place
Cambridge
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03694 - Fussenegger, Martin / Fussenegger, Martin
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03694 - Fussenegger, Martin / Fussenegger, Martin
ethz.date.deposited
2017-06-12T06:43:36Z
ethz.source
ECIT
ethz.identifier.importid
imp5936546e52efc64218
ethz.ecitpid
pub:178773
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-31T12:28:55Z
ethz.rosetta.lastUpdated
2018-11-02T23:20:24Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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