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dc.contributor.author
Sevim, Semih
dc.contributor.author
Shamsudhin, Naveen
dc.contributor.author
Ozer, Sevil
dc.contributor.author
Feng, Luying
dc.contributor.author
Fakhraee, Arielle
dc.contributor.author
Ergeneman, Olgaç
dc.contributor.author
Pané, Salvador
dc.contributor.author
Nelson, Bradley J.
dc.contributor.author
Torun, Hamdi
dc.date.accessioned
2018-10-16T13:04:15Z
dc.date.available
2017-06-12T07:39:30Z
dc.date.available
2018-10-16T13:04:15Z
dc.date.issued
2016-06-06
dc.identifier.other
10.1038/srep27567
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/117463
dc.identifier.doi
10.3929/ethz-b-000117463
dc.description.abstract
We report a modular atomic force microscope (AFM) design for biomolecular experiments. The AFM head uses readily available components and incorporates deflection-based optics and a piezotube-based cantilever actuator. Jetted-polymers have been used in the mechanical assembly, which allows rapid manufacturing. In addition, a FeCo-tipped electromagnet provides high-force cantilever actuation with vertical magnetic fields up to 0.55 T. Magnetic field calibration has been performed with a micro-hall sensor, which corresponds well with results from finite element magnetostatics simulations. An integrated force resolution of 1.82 and 2.98 pN, in air and in DI water, respectively was achieved in 1 kHz bandwidth with commercially available cantilevers made of Silicon Nitride. The controller and user interface are implemented on modular hardware to ensure scalability. The AFM can be operated in different modes, such as molecular pulling or force-clamp, by actuating the cantilever with the available actuators. The electromagnetic and piezoelectric actuation capabilities have been demonstrated in unbinding experiments of the biotin-streptavidin complex.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Biosensors
en_US
dc.subject
Atomic force microscopy
en_US
dc.subject
Applications of AFM
en_US
dc.title
An Atomic Force Microscope with Dual Actuation Capability for Biomolecular Experiments
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Scientific Reports
ethz.journal.volume
6
en_US
ethz.pages.start
27567
en_US
ethz.size
10 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Magnetic Nano Actuators for Quantitative Analysis
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
006751867
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02130 - Dep. Maschinenbau und Verfahrenstechnik / Dep. of Mechanical and Process Eng.::02620 - Inst. f. Robotik u. Intelligente Systeme / Inst. Robotics and Intelligent Systems::03627 - Nelson, Bradley J. / Nelson, Bradley J.
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02130 - Dep. Maschinenbau und Verfahrenstechnik / Dep. of Mechanical and Process Eng.::02620 - Inst. f. Robotik u. Intelligente Systeme / Inst. Robotics and Intelligent Systems::03627 - Nelson, Bradley J. / Nelson, Bradley J.
ethz.grant.agreementno
296679
ethz.grant.fundername
EC
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.program
FP7
ethz.date.deposited
2017-06-12T07:43:28Z
ethz.source
ECIT
ethz.identifier.importid
imp5936547989c4656886
ethz.ecitpid
pub:179370
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-15T20:31:40Z
ethz.rosetta.lastUpdated
2019-01-02T14:35:01Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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