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dc.contributor.author
Harris, C. Jake
dc.contributor.author
Husmann, Dylan
dc.contributor.author
Liu, Wanlu
dc.contributor.author
El Kasmi, Farid
dc.contributor.author
Wang, Haifeng
dc.contributor.author
Papikian, Ashot
dc.contributor.author
Pastor, William A.
dc.contributor.author
Moissiard, Guillaume
dc.contributor.author
Vashisht, Ajay A.
dc.contributor.author
Dangl, Jeffery L.
dc.contributor.author
Wohlschlegel, James A.
dc.contributor.author
Jacobsen, Steven E.
dc.date.accessioned
2018-10-25T16:57:28Z
dc.date.available
2017-06-12T07:57:21Z
dc.date.available
2018-10-25T16:57:28Z
dc.date.issued
2016-05-12
dc.identifier.issn
1553-7390
dc.identifier.issn
1553-7404
dc.identifier.other
10.1371/journal.pgen.1005998
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/117647
dc.identifier.doi
10.3929/ethz-b-000117647
dc.description.abstract
The MORC family of GHKL ATPases are an enigmatic class of proteins with diverse chromatin related functions. In Arabidopsis, AtMORC1, AtMORC2, and AtMORC6 act together in heterodimeric complexes to mediate transcriptional silencing of methylated DNA elements. Here, we studied Arabidopsis AtMORC4 and AtMORC7. We found that, in contrast to AtMORC1,2,6, they act to suppress a wide set of non-methylated protein-coding genes that are enriched for those involved in pathogen response. Furthermore, atmorc4 atmorc7 double mutants show a pathogen response phenotype. We found that AtMORC4 and AtMORC7 form homomeric complexes in vivo and are concentrated in discrete nuclear bodies adjacent to chromocenters. Analysis of an atmorc1,2,4,5,6,7 hextuple mutant demonstrates that transcriptional de-repression is largely uncoupled from changes in DNA methylation in plants devoid of MORC function. However, we also uncover a requirement for MORC in both DNA methylation and silencing at a small but distinct subset of RNA-directed DNA methylation target loci. These regions are characterized by poised transcriptional potential and a low density of sites for symmetric cytosine methylation. These results provide insight into the biological function of MORC proteins in higher eukaryotes.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science (PLoS)
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Arabidopsis AtMORC4 and AtMORC7 Form Nuclear Bodies and Repress a Large Number of Protein-Coding Genes
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
PLoS Genetics
ethz.journal.volume
12
en_US
ethz.journal.issue
5
en_US
ethz.journal.abbreviated
PLoS Genet
ethz.pages.start
e1005998
en_US
ethz.size
22 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
005410305
ethz.publication.place
San Francisco, CA
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-12T08:02:40Z
ethz.source
ECIT
ethz.identifier.importid
imp5936547d2189374253
ethz.ecitpid
pub:179564
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-17T08:26:38Z
ethz.rosetta.lastUpdated
2018-10-25T16:57:32Z
ethz.rosetta.versionExported
true
ethz.COinS
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