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dc.contributor.author
Rampini, Silvana K.
dc.contributor.author
Zbinden, Andrea
dc.contributor.author
Speck, Roberto F.
dc.contributor.author
Bloemberg, Guido V.
dc.date.accessioned
2019-04-26T13:15:19Z
dc.date.available
2017-06-12T08:43:15Z
dc.date.available
2019-04-26T13:15:19Z
dc.date.issued
2016
dc.identifier.issn
1471-2180
dc.identifier.other
10.1186/s12866-016-0752-1
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/118134
dc.identifier.doi
10.3929/ethz-b-000118134
dc.description.abstract
Background Broad-range fungal inter spacer region (ITS) polymerase chain reaction (PCR) has been evaluated for the detection and identification of fungi in clinical specimens from severely immunocompromised patients, but not in non-selected patients. Thus, the aim of this study was to compare the diagnostic performance of ITS PCR with that of fungal culture and to investigate its clinical impact on the diagnosis of fungal infections in non-immunocompromised patients. The corresponding patients’ data were retrieved by detailed medical chart reviews. Results Results from 251 specimens showed a high concordance of 89.6 % for ITS PCR and fungal culture. The analytical sensitivity and specificity of ITS PCR considering culture as gold standard were 87.7 and 90.3 %, respectively, the positive and negative predictive value (PPV and NPV) were 76 and 95.5 %, respectively. Assessing the clinical probability of a fungal infection based on detailed chart reviews, PCR had a clinical sensitivity of 88.9 %, a specificity of 86.3 %, a PPV of 64.0 % and a NPV of 96.6 %. The overall performance of fungal broad-range PCR was similar to that of culture. Conclusions Our data show that, in non-selected and non-immunocompromised patients, the performance of ITS PCR is similar to that of culture for detecting fungal infections, not the least because sensitivity of culture in patients under antifungal treatment is surprisingly high. Compared to culture, PCR has the advantage of a rapid time-to-result (approximately two working days), proper identification of rare pathogens, prompt initiation of a species-targeted antifungal treatment, and prospects for automation.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Antifungal
en_US
dc.subject
Broad-range fungal ITS PCR
en_US
dc.subject
Fungal infection
en_US
dc.subject
Mycosis
en_US
dc.subject
Surgical site infections
en_US
dc.title
Similar efficacy of broad-range ITS PCR and conventional fungal culture for diagnosing fungal infections in non-immunocompromised patients
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
BMC Microbiology
ethz.journal.volume
16
en_US
ethz.journal.abbreviated
BMC microbiol. (Online)
ethz.pages.start
132
en_US
ethz.size
8 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.scopus
ethz.identifier.nebis
004100852
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-12T08:49:16Z
ethz.source
ECIT
ethz.identifier.importid
imp5936548700d4318852
ethz.ecitpid
pub:180076
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-17T08:26:50Z
ethz.rosetta.lastUpdated
2019-04-26T13:15:23Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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