Cross-linking and other structural proteomics techniques: How chemistry is enabling mass spectrometry applications in structural biology

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Author
Leitner, Alexander
Date
2016-08-01Type
- Journal Article
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Abstract
The biological function of proteins is heavily influenced by their structures and their organization into assemblies such as protein complexes and regulatory networks. Mass spectrometry (MS) has been a key enabling technology for high-throughput and comprehensive protein identification and quantification on a proteome-wide scale. Besides these essential contributions, MS can also be used to study higher-order structures of biomacromolecules in a variety of ways. In one approach, intact proteins or protein complexes may be directly probed in the mass spectrometer. Alternatively, various forms of solution-phase chemistry are used to introduce modifications in intact proteins and localizing these modifications by MS analysis at the peptide level is used to derive structural information. Here, I will put a spotlight on the central role of chemistry in such mass spectrometry-based methods that bridge proteomics and structural biology, with a particular emphasis on chemical cross-linking of protein complexes. Show more
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https://doi.org/10.3929/ethz-b-000118978Publication status
publishedJournal / series
Chemical ScienceVolume
Pages / Article No.
Publisher
Royal Society of ChemistryMore
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Citations
Cited 10 times in
Web of Science
Cited 11 times in
Scopus
ETH Bibliography
yes
Altmetrics