Edges of human embryonic stem cell colonies display distinct mechanical properties and differentiation potential
dc.contributor.author
Rosowski, Kathryn A.
dc.contributor.author
Mertz, Aaron F.
dc.contributor.author
Norcross, Samuel
dc.contributor.author
Dufresne, Eric R.
dc.contributor.author
Horsley, Valerie
dc.date.accessioned
2018-10-17T16:57:11Z
dc.date.available
2017-06-12T10:47:23Z
dc.date.available
2018-10-17T16:57:11Z
dc.date.issued
2015-09-22
dc.identifier.issn
2045-2322
dc.identifier.other
10.1038/srep14218
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/119387
dc.identifier.doi
10.3929/ethz-b-000119387
dc.description.abstract
In order to understand the mechanisms that guide cell fate decisions during early human development, we closely examined the differentiation process in adherent colonies of human embryonic stem cells (hESCs). Live imaging of the differentiation process reveals that cells on the outer edge of the undifferentiated colony begin to differentiate first and remain on the perimeter of the colony to eventually form a band of differentiation. Strikingly, this band is of constant width in all colonies, independent of their size. Cells at the edge of undifferentiated colonies show distinct actin organization, greater myosin activity and stronger traction forces compared to cells in the interior of the colony. Increasing the number of cells at the edge of colonies by plating small colonies can increase differentiation efficiency. Our results suggest that human developmental decisions are influenced by cellular environments and can be dictated by colony geometry of hESCs.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Stem-cell niche
en_US
dc.subject
Embryonic stem cells
en_US
dc.subject
Differentiation
en_US
dc.subject
Biological physics
en_US
dc.title
Edges of human embryonic stem cell colonies display distinct mechanical properties and differentiation potential
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Scientific Reports
ethz.journal.volume
5
en_US
ethz.journal.abbreviated
Sci Rep
ethz.pages.start
14218
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.publication.place
London
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02160 - Dep. Materialwissenschaft / Dep. of Materials::09573 - Dufresne, Eric (ehemalig) / Dufresne, Eric (former)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02160 - Dep. Materialwissenschaft / Dep. of Materials::09573 - Dufresne, Eric (ehemalig) / Dufresne, Eric (former)
ethz.date.deposited
2017-06-12T10:48:20Z
ethz.source
ECIT
ethz.identifier.importid
imp593654a01636083815
ethz.ecitpid
pub:181397
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-12T23:52:50Z
ethz.rosetta.lastUpdated
2024-02-02T06:23:44Z
ethz.rosetta.versionExported
true
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