Microfluidic Cell culturing platform for parallelized long-term, high-resolution imaging in combination with impedance spectroscopy
Abstract
We present an integrated microfluidic system, which combines long-term cell culturing with electrical impedance spectroscopy (EIS) on a single chip. Non-adherent cells, such as yeast (S. cerevisiae), can be grown under constant medium perfusion in a 2D layer under clamping pads that enable long-term timelapse microscopy within the same focal plane. The addition of EIS provides a complementary method for continuous in situ analysis of single cells and has been used here to monitor the growth rate of cell colonies in real-time. Show more
Publication status
publishedBook title
19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)Pages / Article No.
Publisher
Chemical and Biological Microsystems SocietyEvent
Subject
Microfluidics; Yeast; Impedance SpectroscopyFunding
289995 - Developing single cell technologies for systems biology (EC)
142440 - The Body on a Chip - Human 3-D tissue analogues in microfluidic systems (SNF)
Notes
Extended abstract. Conference lecture on October 27, 2015.More
Show all metadata
ETH Bibliography
yes
Altmetrics