Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography

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Date
2016Type
- Journal Article
Citations
Cited 63 times in
Web of Science
Cited 61 times in
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ETH Bibliography
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Abstract
Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX. Show more
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https://doi.org/10.3929/ethz-b-000119795Publication status
publishedExternal links
Journal / series
Nature CommunicationsVolume
Pages / Article No.
Publisher
Nature Publishing GroupMore
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Citations
Cited 63 times in
Web of Science
Cited 61 times in
Scopus
ETH Bibliography
yes
Altmetrics