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dc.contributor.author
Nogly, Przemyslaw
dc.contributor.author
Panneels, Valérie
dc.contributor.author
Nelson, Garrett
dc.contributor.author
Gati, Cornelius
dc.contributor.author
Kimura, Tetsunari
dc.contributor.author
Milne, Christopher
dc.contributor.author
Milathianaki, Despina
dc.contributor.author
Kubo, Minoru
dc.contributor.author
Wu, Wenting
dc.contributor.author
Conrad, Chelsie
dc.contributor.author
Coe, Jesse
dc.contributor.author
Bean, Richard
dc.contributor.author
Zhao, Yun
dc.contributor.author
Båth, Petra
dc.contributor.author
Dods, Robert
dc.contributor.author
Harimoorthy, Rajiv
dc.contributor.author
Beyerlein, Kenneth R.
dc.contributor.author
Rheinberger, Jan
dc.contributor.author
James, Daniel
dc.contributor.author
DePonte, Daniel
dc.contributor.author
Li, Chufeng
dc.contributor.author
Sala, Leonardo
dc.contributor.author
Williams, Garth J.
dc.contributor.author
Hunter, Mark S.
dc.contributor.author
Koglin, Jason E.
dc.contributor.author
Berntsen, Peter
dc.contributor.author
Nango, Eriko
dc.contributor.author
Iwata, So
dc.contributor.author
Chapman, Henry N.
dc.contributor.author
Fromme, Petra
dc.contributor.author
Frank, Matthias
dc.contributor.author
Abela, Rafael
dc.contributor.author
Boutet, Sébastien
dc.contributor.author
Barty, Anton
dc.contributor.author
White, Thomas A.
dc.contributor.author
Weierstall, Uwe
dc.contributor.author
Spence, John
dc.contributor.author
Neutze, Richard
dc.contributor.author
Schertler, Gebhard
dc.contributor.author
Standfuss, Jörg
dc.date.accessioned
2018-09-12T14:14:22Z
dc.date.available
2017-06-12T11:28:11Z
dc.date.available
2018-09-12T14:14:22Z
dc.date.issued
2016
dc.identifier.issn
2041-1723
dc.identifier.other
10.1038/ncomms12314
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/119795
dc.identifier.doi
10.3929/ethz-b-000119795
dc.description.abstract
Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2016-08-22
ethz.journal.title
Nature Communications
ethz.journal.volume
7
en_US
ethz.journal.abbreviated
Nat Commun
ethz.pages.start
12314
en_US
ethz.size
9 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
007044158
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-12T11:36:03Z
ethz.source
ECIT
ethz.identifier.importid
imp593654a86434386523
ethz.ecitpid
pub:181829
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T00:00:40Z
ethz.rosetta.lastUpdated
2021-02-15T01:43:49Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Lipidic%20cubic%20phase%20injector%20is%20a%20viable%20crystal%20delivery%20system%20for%20time-resolved%20serial%20crystallography&rft.jtitle=Nature%20Communications&rft.date=2016&rft.volume=7&rft.spage=12314&rft.issn=2041-1723&rft.au=Nogly,%20Przemyslaw&Panneels,%20Val%C3%A9rie&Nelson,%20Garrett&Gati,%20Cornelius&Kimura,%20Tetsunari&rft.genre=article&rft_id=info:doi/10.1038/ncomms12314&
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