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dc.contributor.author
Milias-Argeitis, Andreas
dc.contributor.author
Rullan, Marc
dc.contributor.author
Aoki, Stephanie K.
dc.contributor.author
Buchmann, Peter
dc.contributor.author
Khammash, Mustafa Hani
dc.date.accessioned
2018-09-11T13:21:59Z
dc.date.available
2017-06-12T11:48:00Z
dc.date.available
2018-09-11T13:21:59Z
dc.date.issued
2016
dc.identifier.other
10.1038/ncomms12546
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/119985
dc.identifier.doi
10.3929/ethz-b-000119985
dc.description.abstract
Dynamic control of gene expression can have far-reaching implications for biotechnological applications and biological discovery. Thanks to the advantages of light, optogenetics has emerged as an ideal technology for this task. Current state-of-the-art methods for optical expression control fail to combine precision with repeatability and cannot withstand changing operating culture conditions. Here, we present a novel fully automatic experimental platform for the robust and precise long-term optogenetic regulation of protein production in liquid Escherichia coli cultures. Using a computer-controlled light-responsive two-component system, we accurately track prescribed dynamic green fluorescent protein expression profiles through the application of feedback control, and show that the system adapts to global perturbations such as nutrient and temperature changes. We demonstrate the efficacy and potential utility of our approach by placing a key metabolic enzyme under optogenetic control, thus enabling dynamic regulation of the culture growth rate with potential applications in bacterial physiology studies and biotechnology.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Automated optogenetic feedback control for precise and robust regulation of gene expression and cell growth
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2016-08-26
ethz.journal.title
Nature Communications
ethz.journal.volume
7
en_US
ethz.pages.start
12546
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
007044158
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03921 - Khammash, Mustafa / Khammash, Mustafa
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03921 - Khammash, Mustafa / Khammash, Mustafa
ethz.date.deposited
2017-06-12T11:55:59Z
ethz.source
ECIT
ethz.identifier.importid
imp593654abd13a697943
ethz.ecitpid
pub:182034
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-12T21:18:32Z
ethz.rosetta.lastUpdated
2019-02-03T07:51:34Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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