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dc.contributor.author
Cama, Jehangir
dc.contributor.author
Schaich, Michael
dc.contributor.author
Al Nahas, Kareem
dc.contributor.author
Hernández-Ainsa, Silvia
dc.contributor.author
Pagliara, Stefano
dc.contributor.author
Keyser, Ulrich F.
dc.date.accessioned
2018-10-23T10:45:00Z
dc.date.available
2017-06-12T12:20:15Z
dc.date.available
2018-10-23T10:45:00Z
dc.date.issued
2016-09-08
dc.identifier.other
10.1038/srep32824
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/120305
dc.identifier.doi
10.3929/ethz-b-000120305
dc.description.abstract
Quantifying drug permeability across lipid membranes is crucial for drug development. In addition, reduced membrane permeability is a leading cause of antibiotic resistance in bacteria, and hence there is a need for new technologies that can quantify antibiotic transport across biological membranes. We recently developed an optofluidic assay that directly determines the permeability coefficient of autofluorescent drug molecules across lipid membranes. Using ultraviolet fluorescence microscopy, we directly track drug accumulation in giant lipid vesicles as they traverse a microfluidic device while exposed to the drug. Importantly, our measurement does not require the knowledge of the octanol partition coefficient of the drug – we directly determine the permeability coefficient for the specific drug-lipid system. In this work, we report measurements on a range of fluoroquinolone antibiotics and find that their pH dependent lipid permeability can span over two orders of magnitude. We describe various technical improvements for our assay, and provide a new graphical user interface for data analysis to make the technology easier to use for the wider community.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Permeation and transport
en_US
dc.subject
Drug delivery
en_US
dc.title
Direct Optofluidic Measurement of the Lipid Permeability of Fluoroquinolones
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Scientific Reports
ethz.journal.volume
6
en_US
ethz.pages.start
32824
en_US
ethz.size
7 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
006751867
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-12T12:24:46Z
ethz.source
ECIT
ethz.identifier.importid
imp593654b23aef375548
ethz.ecitpid
pub:182370
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-18T11:46:25Z
ethz.rosetta.lastUpdated
2019-01-02T14:52:32Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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