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dc.contributor.author
Frueh, Florian S.
dc.contributor.author
Körbel, Christina
dc.contributor.author
Gassert, Laura
dc.contributor.author
Müller, Andreas
dc.contributor.author
Gousopoulos, Epameinondas
dc.contributor.author
Lindenblatt, Nicole
dc.contributor.author
Giovanoli, Pietro
dc.contributor.author
Laschke, Matthias W.
dc.contributor.author
Menger, Michael D.
dc.date.accessioned
2018-10-12T15:15:49Z
dc.date.available
2017-06-12T13:53:11Z
dc.date.available
2018-10-12T15:15:49Z
dc.date.issued
2016-10-04
dc.identifier.other
10.1038/srep34673
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/121356
dc.identifier.doi
10.3929/ethz-b-000121356
dc.description.abstract
Secondary lymphedema is a common complication of cancer treatment characterized by chronic limb swelling with interstitial inflammation. The rodent hindlimb is a widely used model for the evaluation of novel lymphedema treatments. However, the assessment of limb volume in small animals is challenging. Recently, high-resolution three-dimensional (3D) imaging modalities have been introduced for rodent limb volumetry. In the present study we evaluated the validity of microcomputed tomography (μCT), magnetic resonance imaging (MRI) and ultrasound in comparison to conventional measuring techniques. For this purpose, acute lymphedema was induced in the mouse hindlimb by a modified popliteal lymphadenectomy. The 4-week course of this type of lymphedema was first assessed in 6 animals. In additional 12 animals, limb volumes were analyzed by μCT, 9.4 T MRI and 30 MHz ultrasound as well as by planimetry, circumferential length and paw thickness measurements. Interobserver correlation was high for all modalities, in particular for μCT analysis (r = 0.975, p < 0.001). Importantly, caliper-measured paw thickness correlated well with μCT (r = 0.861), MRI (r = 0.821) and ultrasound (r = 0.800). Because the assessment of paw thickness represents a time- and cost-effective approach, it may be ideally suited for the quantification of rodent hindlimb lymphedema.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Experimental models of disease
en_US
dc.subject
Oedema
en_US
dc.title
High-resolution 3D volumetry versus conventional measuring techniques for the assessment of experimental lymphedema in the mouse hindlimb
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Scientific Reports
ethz.journal.volume
6
en_US
ethz.pages.start
34673
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.notes
.
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
006751867
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-12T13:54:25Z
ethz.source
ECIT
ethz.identifier.importid
imp593654c4d824826000
ethz.ecitpid
pub:183452
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-18T12:53:53Z
ethz.rosetta.lastUpdated
2019-01-02T14:31:25Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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