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dc.contributor.author
Bar-Yaacov, Dan
dc.contributor.author
Frumkin, Idan
dc.contributor.author
Yashiro, Yuka
dc.contributor.author
Chujo, Takeshi
dc.contributor.author
Ishigami, Yuma
dc.contributor.author
Chemla, Yonatan
dc.contributor.author
Blumberg, Amit
dc.contributor.author
Schlesinger, Orr
dc.contributor.author
Bieri, Philipp
dc.contributor.author
Greber, Basil
dc.contributor.author
Ban, Nenad
dc.contributor.author
Zarivach, Raz
dc.contributor.author
Alfonta, Lital
dc.contributor.author
Pilpel, Yitzhak
dc.contributor.author
Suzuki, Tsutomu
dc.contributor.author
Mishmar, Dan
dc.date.accessioned
2019-06-05T13:27:33Z
dc.date.available
2017-06-12T14:56:43Z
dc.date.available
2019-06-05T13:27:33Z
dc.date.issued
2016-09-15
dc.identifier.issn
1544-9173
dc.identifier.issn
1545-7885
dc.identifier.other
10.1371/journal.pbio.1002557
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/121965
dc.identifier.doi
10.3929/ethz-b-000121965
dc.description.abstract
The mitochondrial ribosome, which translates all mitochondrial DNA (mtDNA)-encoded proteins, should be tightly regulated pre- and post-transcriptionally. Recently, we found RNA-DNA differences (RDDs) at human mitochondrial 16S (large) rRNA position 947 that were indicative of post-transcriptional modification. Here, we show that these 16S rRNA RDDs result from a 1-methyladenosine (m1A) modification introduced by TRMT61B, thus being the first vertebrate methyltransferase that modifies both tRNA and rRNAs. m1A947 is conserved in humans and all vertebrates having adenine at the corresponding mtDNA position (90% of vertebrates). However, this mtDNA base is a thymine in 10% of the vertebrates and a guanine in the 23S rRNA of 95% of bacteria, suggesting alternative evolutionary solutions. m1A, uridine, or guanine may stabilize the local structure of mitochondrial and bacterial ribosomes. Experimental assessment of genome-edited Escherichia coli showed that unmodified adenine caused impaired protein synthesis and growth. Our findings revealed a conserved mechanism of rRNA modification that has been selected instead of DNA mutations to enable proper mitochondrial ribosome function.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
PLOS
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Mitochondrial 16S rRNA Is Methylated by tRNA Methyltransferase TRMT61B in All Vertebrates
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
PLoS Biology
ethz.journal.volume
14
en_US
ethz.journal.issue
9
en_US
ethz.journal.abbreviated
PLoS biol.
ethz.pages.start
e1002557
en_US
ethz.size
21 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.notes
A correction to this paper was published and can be downloaded as a pdf.
en_US
ethz.identifier.scopus
ethz.publication.place
Lawrence, KS
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02521 - Inst. f. Molekularbiologie u. Biophysik / Inst. Molecular Biology and Biophysics::03556 - Ban, Nenad / Ban, Nenad
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02521 - Inst. f. Molekularbiologie u. Biophysik / Inst. Molecular Biology and Biophysics::03556 - Ban, Nenad / Ban, Nenad
ethz.date.deposited
2017-06-12T15:02:22Z
ethz.source
ECIT
ethz.identifier.importid
imp593654d176c7550978
ethz.ecitpid
pub:184228
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-15T05:02:42Z
ethz.rosetta.lastUpdated
2024-02-02T08:12:33Z
ethz.rosetta.versionExported
true
ethz.COinS
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