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dc.contributor.author
Blom, Rebecca A.M.
dc.contributor.author
Erni, Silvia T.
dc.contributor.author
Krempaska, Kristína
dc.contributor.author
Schaerer, Olivier
dc.contributor.author
Van Dijk, R. Maarten
dc.contributor.author
Amacker, Mario
dc.contributor.author
Moser, Christian
dc.contributor.author
Hall, Sean R.R.
dc.contributor.author
von Garnier, Christophe
dc.contributor.author
Blank, Fabian
dc.date.accessioned
2018-08-16T12:14:41Z
dc.date.available
2017-06-12T15:09:20Z
dc.date.available
2018-08-16T12:14:41Z
dc.date.issued
2016-09-29
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0163539
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/122137
dc.identifier.doi
10.3929/ethz-b-000122137
dc.description.abstract
The respiratory tract with its ease of access, vast surface area and dense network of antigen-presenting cells (APCs) represents an ideal target for immune-modulation. Bio-mimetic nanocarriers such as virosomes may provide immunomodulatory properties to treat diseases such as allergic asthma. In our study we employed a triple co-culture model of epithelial cells, macrophages and dendritic cells to simulate the human airway barrier. The epithelial cell line 16HBE was grown on inserts and supplemented with human blood monocyte-derived macrophages (MDMs) and dendritic cells (MDDCs) for exposure to influenza virosomes and liposomes. Additionally, primary human nasal epithelial cells (PHNEC) and EpCAM+ epithelial progenitor cell mono-cultures were utilized to simulate epithelium from large and smaller airways, respectively. To assess particle uptake and phenotype change, cell cultures were analyzed by flow cytometry and pro-inflammatory cytokine concentrations were measured by ELISA. All cell types internalized virosomes more efficiently than liposomes in both mono- and co-cultures. APCs like MDMs and MDDCs showed the highest uptake capacity. Virosome and liposome treatment caused a moderate degree of activation in MDDCs from mono-cultures and induced an increased cytokine production in co-cultures. In epithelial cells, virosome uptake was increased compared to liposomes in both mono- and co-cultures with EpCAM+ epithelial progenitor cells showing highest uptake capacity. In conclusion, all cell types successfully internalized both nanocarriers with virosomes being taken up by a higher proportion of cells and at a higher rate inducing limited activation of MDDCs. Thus virosomes may represent ideal carrier antigen systems to modulate mucosal immune responses in the respiratory tract without causing excessive inflammatory changes.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
A Triple Co-Culture Model of the Human Respiratory Tract to Study Immune-Modulatory Effects of Liposomes and Virosomes
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
PLoS ONE
ethz.journal.volume
11
en_US
ethz.journal.issue
9
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e0163539
en_US
ethz.size
25 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
006206116
ethz.publication.place
Lawrence, KS, USA
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-12T15:11:12Z
ethz.source
ECIT
ethz.identifier.importid
imp593654d4bb9b214861
ethz.ecitpid
pub:184421
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T17:31:54Z
ethz.rosetta.lastUpdated
2018-08-16T12:14:48Z
ethz.rosetta.versionExported
true
ethz.COinS
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