ATPase activity of the DEAD-box protein Dhh1 controls processing body formation

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Author
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Date
2016-10Type
- Journal Article
Citations
Cited 66 times in
Web of Science
Cited 64 times in
Scopus
ETH Bibliography
yes
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Abstract
Translational repression and mRNA degradation are critical mechanisms of posttranscriptional gene regulation that help cells respond to internal and external cues. In response to certain stress conditions, many mRNA decay factors are enriched in processing bodies (PBs), cellular structures involved in degradation and/or storage of mRNAs. Yet, how cells regulate assembly and disassembly of PBs remains poorly understood. Here, we show that in budding yeast, mutations in the DEAD-box ATPase Dhh1 that prevent ATP hydrolysis, or that affect the interaction between Dhh1 and Not1, the central scaffold of the CCR4-NOT complex and an activator of the Dhh1 ATPase, prevent PB disassembly in vivo. Intriguingly, this process can be recapitulated in vitro, since recombinant Dhh1 and RNA, in the presence of ATP, phase-separate into liquid droplets that rapidly dissolve upon addition of Not1. Our results identify the ATPase activity of Dhh1 as a critical regulator of PB formation. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000122727Publication status
publishedExternal links
Journal / series
eLifeVolume
Pages / Article No.
Publisher
eLife Sciences PublicationsOrganisational unit
09464 - Weis, Karsten / Weis, Karsten
Funding
159731 - Structure and Function of the Nuclear Pore Complex (SNF)
More
Show all metadata
Citations
Cited 66 times in
Web of Science
Cited 64 times in
Scopus
ETH Bibliography
yes
Altmetrics