- Journal Article
Rights / licenseCreative Commons Attribution 4.0 International
Starch, as the major nutritional component of our staple crops and a feedstock for industry, is a vital plant product. It is composed of glucose polymers that form massive semi-crystalline granules. Its precise structure and composition determine its functionality and thus applications; however, there is no versatile model system allowing the relationships between the biosynthetic apparatus, glucan structure and properties to be explored. Here, we expressed the core Arabidopsis starch-biosynthesis pathway in Saccharomyces cerevisiae purged of its endogenous glycogen-metabolic enzymes. Systematic variation of the set of biosynthetic enzymes illustrated how each affects glucan structure and solubility. Expression of the complete set resulted in dense, insoluble granules with a starch-like semi-crystalline organization, demonstrating that this system indeed simulates starch biosynthesis. Thus, the yeast system has the potential to accelerate starch research and help create a holistic understanding of starch granule biosynthesis, providing a basis for the targeted biotechnological improvement of crops. Show more
Journal / serieseLife
Pages / Article No.
PublishereLife Sciences Publications
Organisational unit03707 - Zeeman, Samuel C. / Zeeman, Samuel C.
03857 - Mezzenga, Raffaele / Mezzenga, Raffaele
163503 - Designing starch – harnessing carbohydrate polymer synthesis in plants (SNF)
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