Multiple Single-Unit Long-Term Tracking on Organotypic Hippocampal Slices Using High-Density Microelectrode Arrays
Bakkum, Douglas J.
Obien, Marie E.J.
- Journal Article
Rights / licenseCreative Commons Attribution 4.0 International
A novel system to cultivate and record from organotypic brain slices directly on high-density microelectrode arrays (HD-MEA) was developed. This system allows for continuous recording of electrical activity of specific individual neurons at high spatial resolution while monitoring at the same time, neuronal network activity. For the first time, the electrical activity patterns of single neurons and the corresponding neuronal network in an organotypic hippocampal slice culture were studied during several consecutive weeks at daily intervals. An unsupervised iterative spike-sorting algorithm, based on PCA and k-means clustering, was developed to assign the activities to the single units. Spike-triggered average extracellular waveforms of an action potential recorded across neighboring electrodes, termed “footprints” of single-units were generated and tracked over weeks. The developed system offers the potential to study chronic impacts of drugs or genetic modifications on individual neurons in slice preparations over extended times. Show more
Journal / seriesFrontiers in Neuroscience
Pages / Article No.
PublisherFrontiers Research Foundation
SubjectOrganotypic slice; Hippocampus; HD-MEA recording; New device; Neuron tracking
Organisational unit03684 - Hierlemann, Andreas / Hierlemann, Andreas
264417 - Biomedical engineering for cancer and brain disease diagnosis and therapy development (EC)
694829 - Microtechnology and integrated microsystems to investigate neuronal networks across scales (EC)
157092 - Microtechnology and microelectronics to study mammalian axons (SNF)
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