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dc.contributor.author
Gong, Wei
dc.contributor.author
Senčar, Jure
dc.contributor.author
Bakkum, Douglas J.
dc.contributor.author
Jäckel, David
dc.contributor.author
Obien, Marie E.J.
dc.contributor.author
Radivojevic, Milos
dc.contributor.author
Hierlemann, Andreas
dc.date.accessioned
2019-05-29T11:10:24Z
dc.date.available
2017-06-12T17:02:31Z
dc.date.available
2019-05-29T11:10:24Z
dc.date.issued
2016-11-22
dc.identifier.issn
1662-453X
dc.identifier.issn
1662-4548
dc.identifier.other
10.3389/fnins.2016.00537
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/123566
dc.identifier.doi
10.3929/ethz-b-000123566
dc.description.abstract
A novel system to cultivate and record from organotypic brain slices directly on high-density microelectrode arrays (HD-MEA) was developed. This system allows for continuous recording of electrical activity of specific individual neurons at high spatial resolution while monitoring at the same time, neuronal network activity. For the first time, the electrical activity patterns of single neurons and the corresponding neuronal network in an organotypic hippocampal slice culture were studied during several consecutive weeks at daily intervals. An unsupervised iterative spike-sorting algorithm, based on PCA and k-means clustering, was developed to assign the activities to the single units. Spike-triggered average extracellular waveforms of an action potential recorded across neighboring electrodes, termed “footprints” of single-units were generated and tracked over weeks. The developed system offers the potential to study chronic impacts of drugs or genetic modifications on individual neurons in slice preparations over extended times.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers Research Foundation
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Organotypic slice
en_US
dc.subject
Hippocampus
en_US
dc.subject
HD-MEA recording
en_US
dc.subject
New device
en_US
dc.subject
Neuron tracking
en_US
dc.title
Multiple Single-Unit Long-Term Tracking on Organotypic Hippocampal Slices Using High-Density Microelectrode Arrays
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Frontiers in Neuroscience
ethz.journal.volume
10
en_US
ethz.journal.abbreviated
Front Neurosci
ethz.pages.start
537
en_US
ethz.size
16 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Biomedical engineering for cancer and brain disease diagnosis and therapy development
en_US
ethz.grant
Microtechnology and integrated microsystems to investigate neuronal networks across scales
en_US
ethz.grant
Microtechnology and microelectronics to study mammalian axons
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
009497874
ethz.publication.place
Lausanne
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03684 - Hierlemann, Andreas / Hierlemann, Andreas
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03684 - Hierlemann, Andreas / Hierlemann, Andreas
ethz.grant.agreementno
264417
ethz.grant.agreementno
694829
ethz.grant.agreementno
157092
ethz.grant.fundername
EC
ethz.grant.fundername
EC
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
FP7
ethz.grant.program
H2020
ethz.grant.program
Projekte MINT
ethz.date.deposited
2017-06-12T17:03:12Z
ethz.source
ECIT
ethz.identifier.importid
imp593654ee2ee2e16158
ethz.ecitpid
pub:185972
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-26T20:42:43Z
ethz.rosetta.lastUpdated
2022-03-28T23:00:10Z
ethz.rosetta.versionExported
true
ethz.COinS
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