A novel bioreactor system for the assessment of endothelialization on deformable surfaces
Bachmann, Björn J.
- Journal Article
Rights / licenseCreative Commons Attribution 4.0 International
The generation of a living protective layer at the luminal surface of cardiovascular devices, composed of an autologous functional endothelium, represents the ideal solution to life-threatening, implant-related complications in cardiovascular patients. The initial evaluation of engineering strategies fostering endothelial cell adhesion and proliferation as well as the long-term tissue homeostasis requires in vitro testing in environmental model systems able to recapitulate the hemodynamic conditions experienced at the blood-to-device interface of implants as well as the substrate deformation. Here, we introduce the design and validation of a novel bioreactor system which enables the long-term conditioning of human endothelial cells interacting with artificial materials under dynamic combinations of flow-generated wall shear stress and wall deformation. The wall shear stress and wall deformation values obtained encompass both the physiological and supraphysiological range. They are determined through separate actuation systems which are controlled based on validated computational models. In addition, we demonstrate the good optical conductivity of the system permitting online monitoring of cell activities through live-cell imaging as well as standard biochemical post-processing. Altogether, the bioreactor system defines an unprecedented testing hub for potential strategies toward the endothelialization or re-endothelialization of target substrates. Show more
Journal / seriesScientific Reports
Pages / Article No.
PublisherNature Publishing Group
Subjectcardiovascular models; Adherens junctions
Organisational unit03462 - Poulikakos, Dimos / Poulikakos, Dimos
03507 - Ermanni, Paolo / Ermanni, Paolo
03605 - Mazza, Edoardo / Mazza, Edoardo
Related publications and datasets
Is part of: https://doi.org/10.3929/ethz-b-000277918
MoreShow all metadata