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dc.contributor.author
Bammert, Lukas
dc.contributor.author
Jonas, Stefanie
dc.contributor.author
Ungricht, Rosemarie
dc.contributor.author
Kutay, Ulrike
dc.date.accessioned
2019-03-27T17:28:04Z
dc.date.available
2017-06-12T19:08:15Z
dc.date.available
2019-03-27T17:28:04Z
dc.date.issued
2016-11
dc.identifier.issn
1362-4962
dc.identifier.issn
0301-5610
dc.identifier.other
10.1093/nar/gkw790
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/126993
dc.identifier.doi
10.3929/ethz-b-000126993
dc.description.abstract
Mammalian AATF/Che-1 is essential for embryonic development, however, the underlying molecular mechanism is unclear. By immunoprecipitation of human AATF we discovered that AATF forms a salt-stable protein complex together with neuroguidin (NGDN) and NOL10, and demonstrate that the AATF-NGDN-NOL10 (ANN) complex functions in ribosome biogenesis. All three ANN complex members localize to nucleoli and display a mutual dependence with respect to protein stability. Mapping of protein-protein interaction domains revealed the importance of both the evolutionary conserved WD40 repeats in NOL10 and the UTP3/SAS10 domain in NGDN for complex formation. Functional analysis showed that the ANN complex supports nucleolar steps of 40S ribosomal subunit biosynthesis. All complex members were required for 18S rRNA maturation and their individual depletion affected the same nucleolar cleavage steps in the 5΄ETS and ITS1 regions of the ribosomal RNA precursor. Collectively, we identified the ANN complex as a novel functional module supporting the nucleolar maturation of 40S ribosomal subunits. Our data help to explain the described role of AATF in cell proliferation during mouse development as well as its requirement for malignant tumor growth.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Oxford University Press
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc/4.0/
dc.title
Human AATF/Che-1 forms a nucleolar protein complex with NGDN and NOL10 required for 40S ribosomal subunit synthesis
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial 4.0 International
dc.date.published
2016-09-05
ethz.journal.title
Nucleic Acids Research
ethz.journal.volume
44
en_US
ethz.journal.issue
20
en_US
ethz.journal.abbreviated
Nucleic Acids Res.
ethz.pages.start
9803
en_US
ethz.pages.end
9820
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Ribosome synthesis in mammalian cells
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Oxford
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03543 - Kutay, Ulrike / Kutay, Ulrike
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03543 - Kutay, Ulrike / Kutay, Ulrike
ethz.grant.agreementno
166565
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projekte Lebenswissenschaften
ethz.date.deposited
2017-06-12T19:08:55Z
ethz.source
ECIT
ethz.identifier.importid
imp593655293077638205
ethz.ecitpid
pub:189788
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-12T14:33:46Z
ethz.rosetta.lastUpdated
2024-02-02T07:26:59Z
ethz.rosetta.versionExported
true
ethz.COinS
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