Hanging-drop networks for in vitro dose-response studies on 3D tumor microtissues
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This paper presents an analytical platform, based on an inverted, completely open microfluidic system. Interconnected hanging drops form underneath a structured PDMS surface, and 3D tumor microtissues can be cultured in these hanging drops over several days. Continuous perfusion and the integration of a gradient generator enable on-chip dose-response studies. Microscopy readout allows for continuously monitoring the effects of different doses of anti-cancer drugs. Colon cancer microtissues (HCT116) were used to compare the IC50 and IC85 of 5-Fluorouracil (5-FU) under static conditions in a well plate and under perfusion conditions in the hanging-drop network and revealed significant differences. Values of perfused systems more closely matched the reported in vivo doses. Show more
Book title20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Pages / Article No.
PublisherChemical and Biological Microsystems Society
SubjectMicrofluidics; Hanging-drop networks; 3D microtissues; Dose response
Organisational unit03684 - Hierlemann, Andreas / Hierlemann, Andreas
296257 - The Body-on-a-Chip (BoC) (EC)
142440 - The Body on a Chip - Human 3-D tissue analogues in microfluidic systems (SNF)
NotesPoster abstract. Poster presentation on October 11, 2016.
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