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An increasing number of biological and chemical assays have been successfully transferred to droplet-based microfluidic formats. While droplet-based microfluidics typically offers the benefits of high-throughput operation and low reagent consumption, identifying each individual droplet within a large population remains challenging. Several barcoding methods for droplets have been proposed. Herein we use a novel strategy to vastly increase the effective number of barcoded droplets produced, by actively sorting unique fluorescent barcodes from a gradient. Our method is flexible in regard to the number of barcodes, type and number of fluorophores as well as detector sensitivity. In a proof-of-concept experiment we show the production of 2304 uniquely identifiable barcoded droplets. Show more
Book title20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)
Pages / Article No.
PublisherChemical and Biological Microsystems Society
SubjectBarcode; Droplet; Automatic
NotesPoster abstract. Poster presented on October 10, 2016.
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