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Date
2016Type
- Other Conference Item
ETH Bibliography
yes
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Abstract
An increasing number of biological and chemical assays have been successfully transferred to droplet-based microfluidic formats. While droplet-based microfluidics typically offers the benefits of high-throughput operation and low reagent consumption, identifying each individual droplet within a large population remains challenging. Several barcoding methods for droplets have been proposed. Herein we use a novel strategy to vastly increase the effective number of barcoded droplets produced, by actively sorting unique fluorescent barcodes from a gradient. Our method is flexible in regard to the number of barcodes, type and number of fluorophores as well as detector sensitivity. In a proof-of-concept experiment we show the production of 2304 uniquely identifiable barcoded droplets. Show more
Publication status
publishedBook title
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2016)Pages / Article No.
Publisher
Chemical and Biological Microsystems SocietyEvent
Subject
Barcode; Droplet; AutomaticNotes
Poster abstract. Poster presented on October 10, 2016.More
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ETH Bibliography
yes
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