Spatialyse – Spatially precise analysis of nucleic acids using local cell lysis with a microfluidic probe
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We present a method – Spatialyse – to selectively lyse adherent cells for nucleic acid analyses (Fig. 1a) and deliver this lysate in minimum volume for downstream molecular analysis. We make use of a scanning probe technology – a microfluidic probe – and implement hierarchical hydrodynamic flow confinement (hHFC) to localize multiple biochemicals on a biological substrate in a non-contact, non-destructive manner. hHFC enables rapid recovery of nucleic acids by coupling cell lysis and lysate collection. This method can be used for spatio-temporal studies of heterogeneous cells while in culture in contrast to current approaches, providing insight into cellular signaling. Show more
Book title20th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2016)
Pages / Article No.
PublisherChemical and Biological Microsystems Society
SubjectCellular heterogeneity; Nucleic acid analysis; Q-rtPCR; Selective lysis
NotesExtended abstract. Conference lecture on October 10, 2016.
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