An integrated workflow for charting the human interaction proteome: insights into the PP2A system

Open access
Date
2009-01Type
- Journal Article
ETH Bibliography
yes
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Abstract
Protein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP‐MS) yielded a wealth of information on the compendium of protein complexes expressed in Saccharomyces cerevisiae. However, global AP‐MS analysis of human protein complexes is hampered by the low throughput, sensitivity and data robustness of existing procedures, which limit its application for systems biology research. Here, we address these limitations by a novel integrated method, which we applied and benchmarked for the human protein phosphatase 2A system. We identified a total of 197 protein interactions with high reproducibility, showing the coexistence of distinct classes of phosphatase complexes that are linked to proteins implicated in mitosis, cell signalling, DNA damage control and more. These results show that the presented analytical process will substantially advance throughput and reproducibility in future systematic AP‐MS studies on human protein complexes. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000014116Publication status
publishedExternal links
Journal / series
Molecular Systems BiologyVolume
Pages / Article No.
Publisher
NatureSubject
human interactome; mass spectrometry; PP2A; protein complexesOrganisational unit
03663 - Aebersold, Rudolf (emeritus) / Aebersold, Rudolf (emeritus)
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ETH Bibliography
yes
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