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dc.contributor.author
Glatter, Timo
dc.contributor.author
Wepf, Alexander
dc.contributor.author
Aebersold, Ruedi
dc.contributor.author
Gstaiger, Matthias
dc.date.accessioned
2018-06-07T13:20:14Z
dc.date.available
2017-06-08T21:16:30Z
dc.date.available
2018-06-07T13:20:14Z
dc.date.issued
2009-01
dc.identifier.other
10.1038/msb.2008.75
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/14116
dc.identifier.doi
10.3929/ethz-b-000014116
dc.description.abstract
Protein complexes represent major functional units for the execution of biological processes. Systematic affinity purification coupled with mass spectrometry (AP‐MS) yielded a wealth of information on the compendium of protein complexes expressed in Saccharomyces cerevisiae. However, global AP‐MS analysis of human protein complexes is hampered by the low throughput, sensitivity and data robustness of existing procedures, which limit its application for systems biology research. Here, we address these limitations by a novel integrated method, which we applied and benchmarked for the human protein phosphatase 2A system. We identified a total of 197 protein interactions with high reproducibility, showing the coexistence of distinct classes of phosphatase complexes that are linked to proteins implicated in mitosis, cell signalling, DNA damage control and more. These results show that the presented analytical process will substantially advance throughput and reproducibility in future systematic AP‐MS studies on human protein complexes.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publ. Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-sa/3.0/
dc.subject
human interactome
en_US
dc.subject
mass spectrometry
en_US
dc.subject
PP2A
en_US
dc.subject
protein complexes
en_US
dc.title
An integrated workflow for charting the human interaction proteome: insights into the PP2A system
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported
dc.date.published
2009-01-20
ethz.journal.title
Molecular Systems Biology
ethz.journal.volume
5
en_US
ethz.journal.issue
1
en_US
ethz.pages.start
237
en_US
ethz.size
14 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
004931271
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03663 - Aebersold, Rudolf / Aebersold, Rudolf
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03663 - Aebersold, Rudolf / Aebersold, Rudolf
ethz.date.deposited
2017-06-08T21:16:46Z
ethz.source
ECIT
ethz.identifier.importid
imp59364c384115437482
ethz.ecitpid
pub:25667
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-24T09:57:01Z
ethz.rosetta.lastUpdated
2020-02-15T13:27:23Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=An%20integrated%20workflow%20for%20charting%20the%20human%20interaction%20proteome:%20insights%20into%20the%20PP2A%20system&rft.jtitle=Molecular%20Systems%20Biology&rft.date=2009-01&rft.volume=5&rft.issue=1&rft.spage=237&rft.au=Glatter,%20Timo&Wepf,%20Alexander&Aebersold,%20Ruedi&Gstaiger,%20Matthias&rft.genre=article&
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