- Journal Article
Rights / licenseCreative Commons Attribution 3.0 Unported
We have investigated the pathway by which unilamellar POPC liposomes upon adsorption undergo rupture and form a supported lipid bilayer (SLB) on a SiO2 surface. Biotinylated lipids were selectively incorporated in the outer monolayer of POPC liposomes to create liposomes with asymmetric lipid compositions in the outer and inner leaflets. The specific binding of neutravidin and anti-biotin to SLBs formed by liposome fusion, prior to and after equilibrated flip-flop between the upper and lower monolayers in the SLB, were then investigated. It was concluded that the lipids in the outer monolayer of the vesicle predominantly end up on the SLB side facing the SiO2 substrate, as demonstrated by having maximum 30-40% of lipids in the liposome outer monolayer orienting towards the bulk after forming the SLB Show more
Journal / seriesInternational journal of molecular sciences
Pages / Article No.
SubjectLipid Vesicle; Supported Bilayer; Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D); Adsorption; Asymmetric Labeling
Organisational unit03389 - Spencer, Nicholas / Spencer, Nicholas
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