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dc.contributor.author
Reimhult, Erik
dc.contributor.author
Kasemo, Bengt
dc.contributor.author
Höök, Fredrik
dc.date.accessioned
2018-11-05T12:11:36Z
dc.date.available
2017-06-08T21:43:57Z
dc.date.available
2018-11-05T12:11:36Z
dc.date.issued
2009-04-17
dc.identifier.issn
1422-0067
dc.identifier.other
10.3390/ijms10041683
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/15400
dc.identifier.doi
10.3929/ethz-b-000015400
dc.description.abstract
We have investigated the pathway by which unilamellar POPC liposomes upon adsorption undergo rupture and form a supported lipid bilayer (SLB) on a SiO2 surface. Biotinylated lipids were selectively incorporated in the outer monolayer of POPC liposomes to create liposomes with asymmetric lipid compositions in the outer and inner leaflets. The specific binding of neutravidin and anti-biotin to SLBs formed by liposome fusion, prior to and after equilibrated flip-flop between the upper and lower monolayers in the SLB, were then investigated. It was concluded that the lipids in the outer monolayer of the vesicle predominantly end up on the SLB side facing the SiO2 substrate, as demonstrated by having maximum 30-40% of lipids in the liposome outer monolayer orienting towards the bulk after forming the SLB.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
MDPI
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.subject
Lipid Vesicle
en_US
dc.subject
Supported Bilayer
en_US
dc.subject
Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D)
en_US
dc.subject
Adsorption
en_US
dc.subject
Asymmetric Labeling
en_US
dc.title
Rupture Pathway of Phosphatidylcholine Liposomes on Silicon Dioxide
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
International Journal of Molecular Sciences
ethz.journal.volume
10
en_US
ethz.journal.issue
4
en_US
ethz.journal.abbreviated
Int. j. mol. sci.
ethz.pages.start
1683
en_US
ethz.pages.end
1696
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
004068146
ethz.publication.place
S.l.
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02160 - Dep. Materialwissenschaft / Dep. of Materials::02646 - Institut für Polymere / Institute of Polymers::03389 - Spencer, Nicholas / Spencer, Nicholas
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02160 - Dep. Materialwissenschaft / Dep. of Materials::02646 - Institut für Polymere / Institute of Polymers::03389 - Spencer, Nicholas / Spencer, Nicholas
ethz.date.deposited
2017-06-08T21:44:07Z
ethz.source
ECIT
ethz.identifier.importid
imp59364c51662d754631
ethz.ecitpid
pub:27188
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-17T09:35:53Z
ethz.rosetta.lastUpdated
2018-11-05T12:11:40Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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