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dc.contributor.author
Ciampi, Maisa B.
dc.contributor.author
Rosewich Gale, Liane
dc.contributor.author
Macedo Lemos, Eliana G. de
dc.contributor.author
Ceresini, Paulo C.
dc.date.accessioned
2019-10-16T12:14:16Z
dc.date.available
2017-06-08T21:46:57Z
dc.date.available
2019-10-16T12:14:16Z
dc.date.issued
2009-07-17
dc.identifier.issn
1415-4757
dc.identifier.issn
1678-4685
dc.identifier.other
10.1590/S1415-47572009005000063
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/15498
dc.identifier.doi
10.3929/ethz-b-000015498
dc.description.abstract
A series of multilocus sequence-based nuclear DNA markers was developed to infer the phylogeographical history of the Basidiomycetous fungal pathogen Rhizoctonia solani AG-1 IA infecting rice and soybean worldwide. The strategy was based on sequencing of cloned genomic DNA fragments (previously used as RFLP probes) and subsequent screening of fungal isolates to detect single nucleotide polymorphisms (SNPs). Ten primer pairs were designed based on these sequences, which resulted in PCR amplification of 200-320 bp size products and polymorphic sequences in all markers analyzed. By direct sequencing we identified both homokaryon and heterokaryon (i.e. dikaryon) isolates at each marker. Cloning the PCR products effectively estimated the allelic phase from heterokaryotic isolates. Information content varied among markers from 0.5 to 5.9 mutations per 100 bp. Thus, the former RFLP codominant probes were successfully converted into six distinctively variable sequence-based nuclear DNA markers. Rather than discarding low polymorphism loci, the combination of these distinctively variable anonymous nuclear markers would constitute an asset for the unbiased estimate of the phylogeographical parameters such as population sizes and divergent times, providing a more reliable species history that shaped the current population structure of R. solani AG-1 IA.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
SBG
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc/4.0/
dc.subject
Multilocus genotyping
en_US
dc.subject
Polymorphisms
en_US
dc.subject
Allelic discrimination
en_US
dc.subject
Primer design
en_US
dc.title
Distinctively variable sequence-based nuclear DNA markers for multilocus phylogeography of the soybean- and rice-infecting fungal pathogen Rhizoctonia solani AG-1 IA
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial 4.0 International
ethz.journal.title
Genetics and Molecular Biology
ethz.journal.volume
32
en_US
ethz.journal.issue
4
en_US
ethz.journal.abbreviated
Genet. Mol. Biol.
ethz.pages.start
840
en_US
ethz.pages.end
846
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
001979632
ethz.publication.place
Ribeirão Preto
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03516 - McDonald, Bruce / McDonald, Bruce
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03516 - McDonald, Bruce / McDonald, Bruce
ethz.date.deposited
2017-06-08T21:47:06Z
ethz.source
ECIT
ethz.identifier.importid
imp59364c53532e953057
ethz.ecitpid
pub:27299
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-31T15:59:28Z
ethz.rosetta.lastUpdated
2019-10-16T12:14:30Z
ethz.rosetta.versionExported
true
ethz.COinS
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