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dc.contributor.author
Rutishauser, Dorothea
dc.contributor.author
Mertz, Kirsten D.
dc.contributor.author
Moos, Rita
dc.contributor.author
Brunner, Erich
dc.contributor.author
Rülicke, Thomas
dc.contributor.author
Calella, Anna Maria
dc.contributor.author
Aguzzi, Adriano
dc.date.accessioned
2018-08-02T12:10:44Z
dc.date.available
2017-06-14T11:28:57Z
dc.date.available
2018-08-02T12:10:44Z
dc.date.issued
2009-02-11
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0004446
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/156981
dc.identifier.doi
10.3929/ethz-b-000156981
dc.description.abstract
The enumeration of the interaction partners of the cellular prion protein, PrPC, may help clarifying its elusive molecular function. Here we added a carboxy proximal myc epitope tag to PrPC. When expressed in transgenic mice, PrPmyc carried a GPI anchor, was targeted to lipid rafts, and was glycosylated similarly to PrPC. PrPmyc antagonized the toxicity of truncated PrP, restored prion infectibility of PrPC-deficient mice, and was physically incorporated into PrPSc aggregates, indicating that it possessed all functional characteristics of genuine PrPC. We then immunopurified myc epitope-containing protein complexes from PrPmyc transgenic mouse brains. Gentle differential elution with epitope-mimetic decapeptides, or a scrambled version thereof, yielded 96 specifically released proteins. Quantitative mass spectrometry with isotope-coded tags identified seven proteins which co-eluted equimolarly with PrPC and may represent component of a multiprotein complex. Selected PrPC interactors were validated using independent methods. Several of these proteins appear to exert functions in axomyelinic maintenance.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
The Comprehensive Native Interactome of a Fully Functional Tagged Prion Protein
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
4
en_US
ethz.journal.issue
2
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e4446
en_US
ethz.size
13 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.publication.place
Lawrence, Kan.
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung & Wirtschaftsbez. / Domain VP Research & Corporate Relations::02207 - Functional Genomics Center Zürich / Functional Genomics Center Zürich
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung & Wirtschaftsbez. / Domain VP Research & Corporate Relations::02207 - Functional Genomics Center Zürich / Functional Genomics Center Zürich
ethz.date.deposited
2017-06-14T11:31:31Z
ethz.source
ECIT
ethz.identifier.importid
imp59364cde8b53053590
ethz.ecitpid
pub:35243
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-13T08:35:28Z
ethz.rosetta.lastUpdated
2018-11-07T17:41:50Z
ethz.rosetta.versionExported
true
ethz.COinS
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