Inheritance patterns of the response to in vitro doubled haploid induction in perennial ryegrass (Lolium perenne L.)
dc.contributor.author
Begheyn, Rachel F.
dc.contributor.author
Roulund, Niels
dc.contributor.author
Vangsgaard, Kirsten
dc.contributor.author
Kopecký, David
dc.contributor.author
Studer, Bruno
dc.date.accessioned
2017-08-23T15:06:27Z
dc.date.available
2017-08-23T13:53:03Z
dc.date.available
2017-08-23T15:05:33Z
dc.date.available
2017-08-23T15:06:27Z
dc.date.issued
2017-09
dc.identifier.issn
0167-6857
dc.identifier.issn
1573-5044
dc.identifier.other
10.1007/s11240-017-1255-y
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/180249
dc.identifier.doi
10.3929/ethz-b-000180249
dc.description.abstract
The ability to produce doubled haploid (DH) plants has found broad application in research and breeding. For major crop species such as maize (Zea mays L.) and barley (Hordeum vulgare L.), routine large-scale production of DHs has enabled the acceleration of breeding processes, for example through efficient generation of homozygous lines. However, in forage crops such as perennial ryegrass (Lolium perenne L.), low and genotype-specific responses to in vitro anther culture (AC) still limit wide-spread use of DHs. Here, we report the responses of nine bi-parental populations, segregating for microspore embryogenesis and plant regeneration capacity, to an effective AC protocol. Genotypes of exceptionally high androgenic ability, producing over 200 green plants per 100 anthers cultured, could be selected. Continuous and distinctly shaped distributions for the evaluated traits were indicative of quantitative polygenic control and the presence of different alleles in each population. An insignificant association of embryo production with plant regeneration, as well as a low correlation between green and albino plant yield (ρ = 0.20), suggested that different genes influence these traits. The populations evaluated here provide a rich source of alleles needed for the introgression of high levels of androgenic capacity into recalcitrant material. Moreover, this germplasm is ideally suited for use in future genotyping and mapping studies so that the genetic control of androgenic capacity in perennial ryegrass can be elucidated. Ultimately, our results will help to realize the potential of DH induction in one of the world’s most important forage crop species.
en_US
dc.format
application/pdf
dc.language.iso
en
en_US
dc.publisher
Springer
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Androgenic capacity
en_US
dc.subject
Androgenesis
en_US
dc.subject
Anther culture
en_US
dc.subject
Doubled haploid (DH)
en_US
dc.subject
Perennial ryegrass (Lolium perenne L.)
en_US
dc.title
Inheritance patterns of the response to in vitro doubled haploid induction in perennial ryegrass (Lolium perenne L.)
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2017-06-19
ethz.journal.title
Plant Cell, Tissue and Organ Culture
ethz.journal.volume
130
en_US
ethz.journal.issue
3
en_US
ethz.journal.abbreviated
Plant Cell Tiss Organ Cult
ethz.pages.start
667
en_US
ethz.pages.end
679
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Berlin
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03969 - Studer, Bruno / Studer, Bruno
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03969 - Studer, Bruno / Studer, Bruno
en_US
ethz.date.deposited
2017-08-23T13:53:03Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
dc.relation.isnodouble
130336
*
ethz.rosetta.installDate
2017-08-23T15:05:37Z
ethz.rosetta.lastUpdated
2024-02-02T02:23:15Z
ethz.rosetta.versionExported
true
ethz.COinS
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