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dc.contributor.author
Archambaud, Cristel
dc.contributor.author
Sismeiro, Odile
dc.contributor.author
Toedling, Joern
dc.contributor.author
Soubigou, Guillaume
dc.contributor.author
Bécavin, Christophe
dc.contributor.author
Lechat, Pierre
dc.contributor.author
Lebreton, J.
dc.contributor.author
Ciaudo, Constance
dc.contributor.author
Cossart, Pascale
dc.date.accessioned
2017-08-28T08:04:59Z
dc.date.available
2017-06-19T15:31:51Z
dc.date.available
2017-07-06T11:49:56Z
dc.date.available
2017-06-11T02:56:32Z
dc.date.available
2017-07-11T11:21:57Z
dc.date.available
2017-07-11T12:42:26Z
dc.date.available
2017-08-28T08:04:59Z
dc.date.issued
2013-12-10
dc.identifier.issn
2150-7511
dc.identifier.issn
2161-2129
dc.identifier.other
10.1128/mBio.00707-13
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/181121
dc.identifier.doi
10.3929/ethz-b-000163755
dc.description.abstract
The intestinal tract is the largest reservoir of microbes in the human body. The intestinal microbiota is thought to be able to modulate alterations of the gut induced by enteropathogens, thereby maintaining homeostasis. Listeria monocytogenes is the agent of listeriosis, an infection transmitted to humans upon ingestion of contaminated food. Crossing of the intestinal barrier is a critical step of the infection before dissemination into deeper organs. Here, we investigated the role of the intestinal microbiota in the regulation of host protein-coding genes and microRNA (miRNA or miR) expression during Listeria infection. We first established the intestinal miRNA signatures corresponding to the 10 most highly expressed miRNAs in the murine ileum of conventional and germfree mice, noninfected and infected with Listeria. Next, we identified 6 miRNAs whose expression decreased upon Listeria infection in conventional mice. Strikingly, five of these miRNA expression variations (in miR-143, miR- 148a, miR-200b, miR-200c, and miR-378) were dependent on the presence of the microbiota. In addition, as is already known, protein-coding genes were highly affected by infection in both conventional and germfree mice. By crossing bioinformatically the predicted targets of the miRNAs to our whole-genome transcriptomic data, we revealed an miRNA-mRNA network that suggested miRNA-mediated global regulation during intestinal infection. Other recent studies have revealed an miRNA response to either bacterial pathogens or commensal bacteria. In contrast, our work provides an unprecedented insight into the impact of the intestinal microbiota on host transcriptional reprogramming during infection by a human pathogen.
en_US
dc.format
application/pdf
dc.language.iso
en
en_US
dc.publisher
American Society for Microbiology
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-sa/3.0/
dc.title
The Intestinal Microbiota Interferes with the microRNA Response upon Oral Listeria Infection
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported
ethz.journal.title
mBio
ethz.journal.volume
4
en_US
ethz.journal.issue
6
en_US
ethz.pages.start
e00707-13
en_US
ethz.size
10 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.code.ddc
DDC - DDC::5 - Science::570 - Life sciences
en_US
ethz.identifier.scopus
ethz.publication.place
Washington, DC
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::03983 - Ciaudo, Constance / Ciaudo, Constance
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::03983 - Ciaudo, Constance / Ciaudo, Constance
ethz.date.deposited
2017-06-11T02:59:03Z
ethz.source
ECIT
ethz.identifier.importid
imp59365177ea0ee49787
ethz.identifier.importid
imp593651739600928025
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-08-28T07:58:46Z
ethz.rosetta.lastUpdated
2021-02-14T18:32:25Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/77982
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/163755
ethz.COinS
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