A functionalized polydimethyl siloxane chip for solvent-free, temperature actuated solid phase extraction

Abstract

This paper introduces a new approach for solvent-free solid phase extraction on a microfluidic chip. As a proof of concept study, we developed PDMS devices with pillar arrays to support the sorbent. Traditional reverse phase sorbent material was replaced by a thermo-responsive polymer, poly(Nisopropylacrylamide), that enables the extraction and release of biomolecules in aqueous media by varying the temperature. Two functionalization methods of the PDMS surface were investigated. This study is the starting point for future work in the field of environmental analysis involving immunodetection methods.