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dc.contributor.author
Fendt, Sarah-Maria
dc.contributor.author
Buescher, Joerg M.
dc.contributor.author
Rudroff, Florian
dc.contributor.author
Picotti, Paola
dc.contributor.author
Zamboni, Nicola
dc.contributor.author
Sauer, Uwe
dc.date.accessioned
2018-06-07T16:26:50Z
dc.date.available
2017-06-08T22:53:11Z
dc.date.available
2018-06-07T16:26:50Z
dc.date.issued
2010-01
dc.identifier.other
10.1038/msb.2010.11
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/18179
dc.identifier.doi
10.3929/ethz-b-000018179
dc.description.abstract
What is the relationship between enzymes and metabolites, the two major constituents of metabolic networks? We propose three alternative relationships between enzyme capacity and metabolite concentration alterations based on a Michaelis–Menten kinetic; that is enzyme capacities, metabolite concentrations, or both could limit the metabolic reaction rates. These relationships imply different correlations between changes in enzyme capacity and metabolite concentration, which we tested by quantifying metabolite, transcript, and enzyme abundances upon local (single‐enzyme modulation) and global (GCR2 transcription factor mutant) perturbations in Saccharomyces cerevisiae. Our results reveal an inverse relationship between fold‐changes in substrate metabolites and their catalyzing enzymes. These data provide evidence for the hypothesis that reaction rates are jointly limited by enzyme capacity and metabolite concentration. Hence, alteration in one network constituent can be efficiently buffered by converse alterations in the other constituent, implying a passive mechanism to maintain metabolic homeostasis upon perturbations in enzyme capacity.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publ. Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-sa/3.0/
dc.subject
design principle
en_US
dc.subject
metabolic network
en_US
dc.subject
metabolomics
en_US
dc.subject
proteomics
en_US
dc.subject
transcriptome
en_US
dc.title
Tradeoff between enzyme and metabolite efficiency maintains metabolic homeostasis upon perturbations in enzyme capacity
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported
dc.date.published
2010-04-13
ethz.journal.title
Molecular Systems Biology
ethz.journal.volume
6
en_US
ethz.journal.issue
1
en_US
ethz.pages.start
356
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
004931271
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03927 - Picotti, Paola / Picotti, Paola
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03663 - Aebersold, Rudolf / Aebersold, Rudolf
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03713 - Sauer, Uwe / Sauer, Uwe
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03927 - Picotti, Paola / Picotti, Paola
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03663 - Aebersold, Rudolf / Aebersold, Rudolf
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03713 - Sauer, Uwe / Sauer, Uwe
ethz.date.deposited
2017-06-08T22:53:16Z
ethz.source
ECIT
ethz.identifier.importid
imp59364c8a18d8c36204
ethz.ecitpid
pub:30292
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-18T15:14:44Z
ethz.rosetta.lastUpdated
2018-11-07T11:31:36Z
ethz.rosetta.versionExported
true
ethz.COinS
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