Show simple item record

dc.contributor.author
Merz, David
dc.contributor.author
Richter, Julia
dc.contributor.author
Gonneau, Martine
dc.contributor.author
Sánchez-Rodríguez, Clara
dc.contributor.author
Eder, Tobias
dc.contributor.author
Sormani, Rodnay
dc.contributor.author
Martin, Marjolaine
dc.contributor.author
Hématy, Kian
dc.contributor.author
Höfte, Herman
dc.contributor.author
Hauser, Marie-Theres
dc.date.accessioned
2018-06-05T10:58:11Z
dc.date.available
2017-09-20T07:47:27Z
dc.date.available
2017-09-20T12:17:21Z
dc.date.available
2018-06-05T10:58:11Z
dc.date.issued
2017-10-03
dc.identifier.other
10.1093/jxb/erx263
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/185885
dc.identifier.doi
10.3929/ethz-b-000185885
dc.description.abstract
Perturbation of cellulose synthesis in plants triggers stress responses, including growth retardation, mediated by the cell wall integrity-sensing receptor-like kinase (RLK) THESEUS1 (THE1). The analysis of two alleles carrying T-DNA insertions at comparable positions has led to conflicting conclusions concerning the impact of THE1 signaling on growth. Here we confirm that, unlike the1-3 and other the1 alleles in which cellular responses to genetic or pharmacological inhibition of cellulose synthesis are attenuated, the1-4 showed enhanced responses, including growth inhibition, ectopic lignification, and stress gene expression. Both the1-3 and the1-4 express a transcript encoding a predicted membrane-associated truncated protein lacking the kinase domain. However, the1-3, in contrast to the1-4, strongly expresses antisense transcripts, which are expected to prevent the expression of the truncated protein as suggested by the genetic interactions between the two alleles. Seedlings overexpressing such a truncated protein react to isoxaben treatment similarly to the1-4 and the full-length THE overexpressor. We conclude that the1-4 is a hypermorphic allele; that THE1 signaling upon cell wall damage has a negative impact on cell expansion; and that caution is required when interpreting the phenotypic effects of T-DNA insertions in RLK genes.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Oxford University Press
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Cell elongation
en_US
dc.subject
Cellulose synthesis
en_US
dc.subject
Cell wall integrity signaling
en_US
dc.subject
CrRLK1L receptor
en_US
dc.subject
Gene silencing
en_US
dc.subject
Isoxaben
en_US
dc.title
T-DNA alleles of the receptor kinase THESEUS1 with opposing effects on cell wall integrity signaling
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2017-08-01
ethz.journal.title
Journal of experimental botany
ethz.journal.volume
68
en_US
ethz.journal.issue
16
en_US
ethz.pages.start
4583
en_US
ethz.pages.end
4593
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.code.ddc
5 - Science::580 - Botanical sciences
en_US
ethz.identifier.wos
ethz.publication.place
Oxford
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::09530 - Sánchez Rodríguez, Clara / Sánchez Rodríguez, Clara
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::09530 - Sánchez Rodríguez, Clara / Sánchez Rodríguez, Clara
en_US
ethz.date.deposited
2017-09-20T07:47:28Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-06-05T10:58:55Z
ethz.rosetta.lastUpdated
2019-02-03T02:02:12Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=T-DNA%20alleles%20of%20the%20receptor%20kinase%20THESEUS1%20with%20opposing%20effects%20on%20cell%20wall%20integrity%20signaling&rft.jtitle=Journal%20of%20experimental%20botany&rft.date=2017-10-03&rft.volume=68&rft.issue=16&rft.spage=4583&rft.epage=4593&rft.au=Merz,%20David&Richter,%20Julia&Gonneau,%20Martine&S%C3%A1nchez-Rodr%C3%ADguez,%20Clara&Eder,%20Tobias&rft.genre=article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record