Golgi-localized STELLO proteins regulate the assembly and trafficking of cellulose synthase complexes in Arabidopsis

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Author
Zhang, Yi
Nikolovski, Nino
Sorieul, Mathias
Vellosillo, Tamara
McFarlane, Heather E.
Dupree, Ray
Kesten, Christopher
Schneider, René
Driemeier, Carlos
Lathe, Rahul
Lampugnani, Edwin
Yu, Xiaolan
Ivakov, Alexander
Doblin, Monika S.
Mortimer, Jenny C.
Brown, Steven P.
Persson, Staffan
Dupree, Paul
Date
2016Type
- Journal Article
ETH Bibliography
no
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Abstract
As the most abundant biopolymer on Earth, cellulose is a key structural component of the plant cell wall. Cellulose is produced at the plasma membrane by cellulose synthase (CesA) complexes (CSCs), which are assembled in the endomembrane system and trafficked to the plasma membrane. While several proteins that affect CesA activity have been identified, components that regulate CSC assembly and trafficking remain unknown. Here we show that STELLO1 and 2 are Golgi-localized proteins that can interact with CesAs and control cellulose quantity. In the absence of STELLO function, the spatial distribution within the Golgi, secretion and activity of the CSCs are impaired indicating a central role of the STELLO proteins in CSC assembly. Point mutations in the predicted catalytic domains of the STELLO proteins indicate that they are glycosyltransferases facing the Golgi lumen. Hence, we have uncovered proteins that regulate CSC assembly in the plant Golgi apparatus. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000186646Publication status
publishedJournal / series
Nature CommunicationsVolume
Pages / Article No.
Publisher
Nature Publishing GroupSubject
Golgi; Protein trafficking in plants; TransferasesOrganisational unit
09530 - Sánchez Rodríguez, Clara / Sánchez Rodríguez, Clara
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ETH Bibliography
no
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