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dc.contributor.author
Shikuma, Nicholas J.
dc.contributor.author
Antoshechkin, Igor
dc.contributor.author
Medeiros, João M.
dc.contributor.author
Pilhofer, Martin
dc.contributor.author
Newman, Dianne K.
dc.date.accessioned
2017-10-04T13:38:49Z
dc.date.available
2017-10-04T13:38:49Z
dc.date.available
2017-06-29T07:03:14Z
dc.date.available
2017-06-28T21:16:11Z
dc.date.available
2017-06-12T11:58:56Z
dc.date.issued
2016-09-06
dc.identifier.other
10.1073/pnas.1603142113
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/189756
dc.identifier.doi
10.3929/ethz-b-000189756
dc.description.abstract
Diverse animal taxa metamorphose between larval and juvenile phases in response to bacteria. Although bacteria-induced metamorphosis is widespread among metazoans, little is known about the molecular changes that occur in the animal upon stimulation by bacteria. Larvae of the tubeworm Hydroides elegans metamorphose in response to surface-bound Pseudoalteromonas luteoviolacea bacteria, producing ordered arrays of phage tail-like metamorphosis-associated contractile structures (MACs). Sequencing the Hydroides genome and transcripts during five developmental stages revealed that MACs induce the regulation of groups of genes important for tissue remodeling, innate immunity, and mitogen-activated protein kinase (MAPK) signaling. Using two MAC mutations that block P. luteoviolacea from inducing settlement or metamorphosis and three MAPK inhibitors, we established a sequence of bacteria-induced metamorphic events: MACs induce larval settlement; then, particular properties of MACs encoded by a specific locus in P. luteoviolacea initiate cilia loss and activate metamorphosis-associated transcription; finally, signaling through p38 and c-Jun N-terminal kinase (JNK) MAPK pathways alters gene expression and leads to morphological changes upon initiation of metamorphosis. Our results reveal that the intricate interaction between Hydroides and P. luteoviolacea can be dissected using genomic, genetic, and pharmacological tools. Hydroides' dependency on bacteria for metamorphosis highlights the importance of external stimuli to orchestrate animal development. The conservation of Hydroides genome content with distantly related deuterostomes (urchins, sea squirts, and humans) suggests that mechanisms of bacteria-induced metamorphosis in Hydroides may have conserved features in diverse animals. As a major biofouling agent, insight into the triggers of Hydroides metamorphosis might lead to practical strategies for fouling control.
en_US
dc.language.iso
en
en_US
dc.publisher
National Academy of Sciences
dc.rights.uri
http://rightsstatements.org/page/InC-NC/1.0/
dc.subject
Biofouling
dc.subject
Development
dc.subject
Genome
dc.subject
Phage
dc.subject
Symbiosis
dc.title
Stepwise metamorphosis of the tubewormHydroides elegansis mediated by a bacterial inducer and MAPK signaling
en_US
dc.type
Journal Article
dc.rights.license
In Copyright - Non-Commercial Use Permitted
dc.date.published
2016-08-22
ethz.journal.title
Proceedings of the National Academy of Sciences of the United States of America
ethz.journal.volume
113
ethz.journal.issue
36
en_US
ethz.pages.start
10097
ethz.pages.end
10102
en_US
ethz.size
28 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
001003347
ethz.publication.place
Washington, DC
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich, direkt::00012 - Lehre und Forschung, direkt::00007 - Departemente, direkt::02030 - Departement Biologie / Department of Biology::02521 - Institut für Molekularbiologie und Biophysik (IMB) / Institute of Molecular Biology and Biophysics (IMB)::09463 - Pilhofer, Martin
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich, direkt::00012 - Lehre und Forschung, direkt::00007 - Departemente, direkt::02030 - Departement Biologie / Department of Biology::02521 - Institut für Molekularbiologie und Biophysik (IMB) / Institute of Molecular Biology and Biophysics (IMB)::09463 - Pilhofer, Martin
en_US
ethz.date.deposited
2017-06-12T12:07:02Z
ethz.source
FORM
ethz.source
ECIT
ethz.identifier.importid
imp593654adc42e988457
ethz.ecitpid
pub:182134
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-10-04T13:43:41Z
ethz.rosetta.lastUpdated
2017-10-04T13:43:41Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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