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dc.contributor.author
Dermutz, Harald
dc.contributor.author
Thompson-Steckel, Greta
dc.contributor.author
Forró, Csaba
dc.contributor.author
de Lange, Victoria
dc.contributor.author
Dorwling-Carter, Livie
dc.contributor.author
Vörös, Janos
dc.contributor.author
Demkó, László
dc.date.accessioned
2017-12-05T13:57:18Z
dc.date.available
2017-10-06T01:54:26Z
dc.date.available
2017-10-23T14:30:30Z
dc.date.available
2017-12-05T13:52:56Z
dc.date.available
2017-12-05T13:57:18Z
dc.date.issued
2017
dc.identifier.issn
2046-2069
dc.identifier.other
10.1039/c7ra00971b
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/190276
dc.identifier.doi
10.3929/ethz-b-000190276
dc.description.abstract
Cells in vitro behave differently if cultured in a 2D or 3D environment. In spite of the continuous progress over the recent years, methods available for realizing 3D cultures of primary neurons are still fairly complex, limited in throughput and especially limited in compatibility with other techniques like multielectrode arrays (MEAs) for recording and stimulating the network activity with high temporal precision. In this manuscript, a paper-based approach is presented using cellulose filter paper as a mobile substrate for 3D cultures of primary rat hippocampal and cortical neurons. Acting as 3D scaffolds for network development, filter membranes with different surface treatments were prepared to control network homogeneity and laser cut to change the network topology through spatial confinement. The viability of the prepared cultures was comparable to that of reference 2D cultures for over 4 weeks, and the mechanical stability of the paper substrates made it possible to transfer the cultures to MEA chips in an on-demand manner. Once the cultures were successfully transduced with a gene-encoded calcium indicator and transferred to a MEA chip, the optical and electrical signals of neuronal activity were simultaneously recorded and combined to study the different activity patterns with high spatiotemporal resolution. The high-throughput nature of the presented approach makes it a valuable tool for investigating the intimate relationship between topology and function, by studying the intrinsic parameters influencing network synchronization and signal propagation through the different activity patterns of 3D neural cultures with arbitrary topology. The developed platform provides a robust and simple alternative to existing 3D culturing technologies for neurons.
en_US
dc.format
application/pdf
dc.language.iso
en
en_US
dc.publisher
Royal Society of Chemistry
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Paper-based patterned 3D neural cultures as a tool to study network activity on multielectrode arrays
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
RSC Advances
ethz.journal.volume
7
en_US
ethz.journal.issue
62
en_US
ethz.journal.abbreviated
RSC Adv.
ethz.pages.start
39359
en_US
ethz.pages.end
39371
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Biomimiking the brain - towards 3D neuronal network dynamics
en_US
ethz.identifier.wos
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.grant.agreementno
296590
ethz.grant.fundername
EC
ethz.grant.funderDoi
10.13039/501100000780
ethz.grant.program
FP7
ethz.date.deposited
2017-10-06T01:54:37Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-10-23T14:30:33Z
ethz.rosetta.lastUpdated
2022-03-28T18:31:25Z
ethz.rosetta.versionExported
true
ethz.COinS
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