Combination of High-density Microelectrode Array and Patch Clamp Recordings to Enable Studies of Multisynaptic Integration

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Date
2017-04-20Type
- Journal Article
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Cited 39 times in
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Cited 37 times in
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Abstract
We present a novel, all-electric approach to record and to precisely control the activity of tens of individual presynaptic neurons. The method allows for parallel mapping of the efficacy of multiple synapses and of the resulting dynamics of postsynaptic neurons in a cortical culture. For the measurements, we combine an extracellular high-density microelectrode array, featuring 11’000 electrodes for extracellular recording and stimulation, with intracellular patch-clamp recording. We are able to identify the contributions of individual presynaptic neurons - including inhibitory and excitatory synaptic inputs - to postsynaptic potentials, which enables us to study dendritic integration. Since the electrical stimuli can be controlled at microsecond resolution, our method enables to evoke action potentials at tens of presynaptic cells in precisely orchestrated sequences of high reliability and minimum jitter. We demonstrate the potential of this method by evoking short- and long-term synaptic plasticity through manipulation of multiple synaptic inputs to a specific neuron. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000190298Publication status
publishedExternal links
Journal / series
Scientific ReportsVolume
Pages / Article No.
Publisher
Nature Publishing GroupOrganisational unit
03684 - Hierlemann, Andreas / Hierlemann, Andreas
Funding
267351 - Seamless Integration of Neurons with CMOS Microelectronics (EC)
694829 - Microtechnology and integrated microsystems to investigate neuronal networks across scales (EC)
157092 - Microtechnology and microelectronics to study mammalian axons (SNF)
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Show all metadata
Citations
Cited 39 times in
Web of Science
Cited 37 times in
Scopus
ETH Bibliography
yes
Altmetrics