Show simple item record

dc.contributor.author
Jomaa, Ahmad
dc.contributor.author
Fu, Yu-Hsien H.
dc.contributor.author
Boehringer, Daniel
dc.contributor.author
Leibundgut, Marc
dc.contributor.author
Shan, Shu-ou
dc.contributor.author
Ban, Nenad
dc.date.accessioned
2017-11-16T15:43:39Z
dc.date.available
2017-10-06T02:14:23Z
dc.date.available
2017-11-16T15:43:39Z
dc.date.issued
2017
dc.identifier.other
10.1038/ncomms15470
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/190577
dc.identifier.doi
10.3929/ethz-b-000190577
dc.description.abstract
During co-translational protein targeting, the signal recognition particle (SRP) binds to the translating ribosome displaying the signal sequence to deliver it to the SRP receptor (SR) on the membrane, where the signal peptide is transferred to the translocon. Using electron cryo-microscopy, we have determined the structure of a quaternary complex of the translating Escherichia coli ribosome, the SRP–SR in the ‘activated’ state and the translocon. Our structure, supported by biochemical experiments, reveals that the SRP RNA adopts a kinked and untwisted conformation to allow repositioning of the ‘activated’ SRP–SR complex on the ribosome. In addition, we observe the translocon positioned through interactions with the SR in the vicinity of the ribosome exit tunnel where the signal sequence is extending beyond its hydrophobic binding groove of the SRP M domain towards the translocon. Our study provides new insights into the mechanism of signal sequence transfer from the SRP to the translocon.
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Structure of the quaternary complex between SRP, SR, and translocon bound to the translating ribosome
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2017-05-19
ethz.journal.title
Nature Communications
ethz.journal.volume
8
en_US
ethz.pages.start
15470
en_US
ethz.size
9 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02521 - Inst. f. Molekularbiologie u. Biophysik / Inst. Molecular Biology and Biophysics::03556 - Ban, Nenad / Ban, Nenad
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02521 - Inst. f. Molekularbiologie u. Biophysik / Inst. Molecular Biology and Biophysics::03556 - Ban, Nenad / Ban, Nenad
ethz.date.deposited
2017-10-06T02:14:30Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-11-16T15:43:43Z
ethz.rosetta.lastUpdated
2019-02-02T13:28:09Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Structure%20of%20the%20quaternary%20complex%20between%20SRP,%20SR,%20and%20translocon%20bound%20to%20the%20translating%20ribosome&rft.jtitle=Nature%20Communications&rft.date=2017&rft.volume=8&rft.spage=15470&rft.au=Jomaa,%20Ahmad&Fu,%20Yu-Hsien%20H.&Boehringer,%20Daniel&Leibundgut,%20Marc&Shan,%20Shu-ou&rft.genre=article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record